Exogenous modulation of intrinsic optic nerve neuroprotective activity

dc.contributor.author Grozdanic, Sinisa
dc.contributor.author Lazic, Tatjana
dc.contributor.author Kuehn, Markus
dc.contributor.author Harper, Matthew
dc.contributor.author Kardon, Randy
dc.contributor.author Kwon, Young
dc.contributor.author Lavik, Erin
dc.contributor.author Sakaguchi, Donald
dc.contributor.department Veterinary Clinical Sciences
dc.contributor.department Neuroscience
dc.contributor.department Genetics, Development and Cell Biology
dc.date 2018-02-18T12:46:01.000
dc.date.accessioned 2020-06-30T04:01:28Z
dc.date.available 2020-06-30T04:01:28Z
dc.date.issued 2010-08-01
dc.description.abstract <p><h3>Background</h3></p> <p>To characterize the molecular and functional status of the rat retina and optic nerve after acute elevation of intraocular pressure (IOP). <h3>Methods</h3></p> <p>Retinal ischemia was induced in rats by increasing the IOP (110 mmHg/60 minutes). Microarray analysis, quantitative RT-PCR (qRT-PCR) and immunohistochemistry were used to characterize retinal tissue. PLGA microspheres containing neurotrophic factors (BDNF, GDNF, or CNTF) or empty microspheres were injected into the vitreous of operated animals 1 day after elevation of IOP. Pupil light reflex (PLR) parameters and electroretinograms (ERG) were monitored at multiple time points during the 60-day postoperative recovery period. <h3>Results</h3></p> <p>Molecular analysis showed a significant intrinsic up-regulation of CNTF at 10 and 25 days after induction of the acute ocular hypertension (<em>p</em> = 0.0067). Molecular tissue analysis of GDNF and its receptors (GDNFR1, GDNFR2), and BDNF and its receptor (trkB) showed no change in expression. Animals that received CNTF microspheres had no significant functional recovery compared to animals which received blank microspheres (<em>p</em> > 0.05). Animals that received GDNF or BDNF microspheres showed significant PLR recovery (<em>p</em> < 0.05 and <em>p</em> < 0.001 respectively) compared to non-treated animals. <h3>Conclusions</h3></p> <p>Continuous release of neurotrophic growth factors (NGFs) significantly protects optic nerve function in the experimental model of retinal ischemia observed by PLR analysis.</p>
dc.description.comments <p>This article is from <em>Graefe's Archive for Clinical and Experimental Ophthalmology </em>248 (2010): 1105, doi: <a href="http://dx.doi.org/10.1007/s00417-010-1336-7" target="_blank">10.1007/s00417-010-1336-7</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/gdcb_las_pubs/155/
dc.identifier.articleid 1158
dc.identifier.contextkey 10229554
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath gdcb_las_pubs/155
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/37823
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/gdcb_las_pubs/155/2010_Sakaguchi_ExogenusModulation.pdf|||Fri Jan 14 20:41:58 UTC 2022
dc.source.uri 10.1007/s00417-010-1336-7
dc.subject.disciplines Cell and Developmental Biology
dc.subject.disciplines Comparative and Laboratory Animal Medicine
dc.subject.disciplines Neuroscience and Neurobiology
dc.subject.keywords Neuroprotection
dc.subject.keywords Retina
dc.subject.keywords BDNF
dc.subject.keywords GDNF
dc.subject.keywords CNTF
dc.title Exogenous modulation of intrinsic optic nerve neuroprotective activity
dc.type article
dc.type.genre article
dspace.entity.type Publication
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