Analysis of Gene Expression in a Region Associated with Host Response to Porcine Reproductive and Respiratory Syndrome Virus Challenge Eisley, Christopher Fritz-Waters, Eric Reecy, James Choi, Igseo Koltes, James Boddicker, Nicholas Reecy, James Lunney, Joan Carpenter, Susan Tuggle, Chris Liu, Peng Dekkers, Jack 2018-08-25T20:26:40.000 2020-06-29T23:35:24Z 2020-06-29T23:35:24Z 2014-02-10 2014-01-01
dc.description.abstract <p>Previous work identified a 1 Megabasepair region encompassing a quantitative trait locus (QTL) on Sus scrofa chromosome 4 associated with response to PRRS virus infection, in terms of weight gain and PRRS viremia. To identify candidate genes in the region responsible for these effects, we evaluated the expression of genes in the region by RNAseq using RNA isolated from whole blood taken from 8 pairs of littermates at 5 time-points following experimental infection with the PRRS virus. Each littermate pair included one individual with the favorable genotype (AB) for the SSC4 region and one with the unfavorable genotype (AA). A comparison of the gene transcript counts between AB and AA individuals was performed with a statistical model that also used sequence information to identify transcripts with allele-specific expression (A versus B) in the AB individuals. These analyses revealed a few candidate genes, with one gene in particular being differentially expressed at multiple time-points and having a high level of overall gene expression. This candidate gene is promising for follow-up functional biology studies to validate and detail its role in host response to PRRS virus infection.</p>
dc.identifier archive/
dc.identifier.articleid 2028
dc.identifier.contextkey 5098200
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_air/vol660/iss1/84
dc.language.iso en
dc.relation.ispartofseries Animal Science Research Reports
dc.relation.ispartofseries ASL R2919
dc.source.bitstream archive/|||Sat Jan 15 02:10:41 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Biostatistics
dc.subject.disciplines Genetics
dc.subject.keywords Animal Science
dc.subject.keywords Statistics
dc.title Analysis of Gene Expression in a Region Associated with Host Response to Porcine Reproductive and Respiratory Syndrome Virus Challenge
dc.type article
dc.type.genre swine
dspace.entity.type Publication
relation.isAuthorOfPublication fb994cd9-94d5-4370-94ab-f33934c4cd6f
relation.isJournalIssueOfPublication 7e474e6a-85cb-446c-9f53-8afe83d5a741
relation.isSeriesOfPublication 7f3839b7-b833-4418-a6fa-adda2b23950a
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