TALE activation and functional analysis of endogenous genes in Chlamydomonas reinhardtii

dc.contributor.advisor Martin H. Spalding
dc.contributor.author Gao, Han
dc.contributor.department Genetics, Development and Cell Biology
dc.date 2018-08-11T12:15:29.000
dc.date.accessioned 2020-06-30T02:54:39Z
dc.date.available 2020-06-30T02:54:39Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2014
dc.date.embargo 2015-08-26
dc.date.issued 2014-01-01
dc.description.abstract <p>Transcription activator-like effectors (TALEs) are effector proteins secreted by certain plant pathogenic bacteria when infecting their hosts. Upon translocation, TALEs bind via a well-deciphered recognition code to specific sequences in the promoter region of targeted host genes, thereby activating expression of those targeted genes. Gene activation induced by artificially designed TALEs (dTALEs) has been studied in multiple organisms, but is yet to be demonstrated in green algae, such as Chlamydomonas reinhardtii, a well-known model organism for fundamental biological studies, as well as for biofuel production. Our work demonstrates robust gene-specific activation induced by artificially designed TALEs in the green alga Chlamydomonas. The frequency and efficiency of the induced expression demonstrate dTALEs as powerful tools for targeted gene activation in Chlamydomonas. The pattern of activation shown here provides insights into the mechanism of TALE induced expression, and also confirms the activity of the activation domain of naturally occurring TALEs in another organism. Our success with dTALE-induced activation in Chlamydomonas may open a new avenue to fast, high throughput gene manipulation in other related organisms, such as green algae and crop plants.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/14142/
dc.identifier.articleid 5149
dc.identifier.contextkey 7738340
dc.identifier.doi https://doi.org/10.31274/etd-180810-3690
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/14142
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/28328
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/14142/Gao_iastate_0097E_14509.pdf|||Fri Jan 14 20:15:04 UTC 2022
dc.subject.disciplines Genetics
dc.subject.keywords Genetics
dc.title TALE activation and functional analysis of endogenous genes in Chlamydomonas reinhardtii
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
thesis.degree.discipline Genetics
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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