TALE activation and functional analysis of endogenous genes in Chlamydomonas reinhardtii

dc.contributor.advisor	Martin H. Spalding
dc.contributor.author	Gao, Han
dc.contributor.department	Genetics, Development and Cell Biology
dc.date	2018-08-11T12:15:29.000
dc.date.accessioned	2020-06-30T02:54:39Z
dc.date.available	2020-06-30T02:54:39Z
dc.date.copyright	Wed Jan 01 00:00:00 UTC 2014
dc.date.embargo	2015-08-26
dc.date.issued	2014-01-01
dc.description.abstract	<p>Transcription activator-like effectors (TALEs) are effector proteins secreted by certain plant pathogenic bacteria when infecting their hosts. Upon translocation, TALEs bind via a well-deciphered recognition code to specific sequences in the promoter region of targeted host genes, thereby activating expression of those targeted genes. Gene activation induced by artificially designed TALEs (dTALEs) has been studied in multiple organisms, but is yet to be demonstrated in green algae, such as Chlamydomonas reinhardtii, a well-known model organism for fundamental biological studies, as well as for biofuel production. Our work demonstrates robust gene-specific activation induced by artificially designed TALEs in the green alga Chlamydomonas. The frequency and efficiency of the induced expression demonstrate dTALEs as powerful tools for targeted gene activation in Chlamydomonas. The pattern of activation shown here provides insights into the mechanism of TALE induced expression, and also confirms the activity of the activation domain of naturally occurring TALEs in another organism. Our success with dTALE-induced activation in Chlamydomonas may open a new avenue to fast, high throughput gene manipulation in other related organisms, such as green algae and crop plants.</p>
dc.format.mimetype	application/pdf
dc.identifier	archive/lib.dr.iastate.edu/etd/14142/
dc.identifier.articleid	5149
dc.identifier.contextkey	7738340
dc.identifier.doi	https://doi.org/10.31274/etd-180810-3690
dc.identifier.s3bucket	isulib-bepress-aws-west
dc.identifier.submissionpath	etd/14142
dc.identifier.uri	https://dr.lib.iastate.edu/handle/20.500.12876/28328
dc.language.iso	en
dc.source.bitstream	archive/lib.dr.iastate.edu/etd/14142/Gao_iastate_0097E_14509.pdf\|\|\|Fri Jan 14 20:15:04 UTC 2022
dc.subject.disciplines	Genetics
dc.subject.keywords	Genetics
dc.title	TALE activation and functional analysis of endogenous genes in Chlamydomonas reinhardtii
dc.type	article
dc.type.genre	dissertation
dspace.entity.type	Publication
relation.isOrgUnitOfPublication	9e603b30-6443-4b8e-aff5-57de4a7e4cb2
thesis.degree.discipline	Genetics
thesis.degree.level	dissertation
thesis.degree.name	Doctor of Philosophy

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