Detecting molecular properties by various laser-based techniques

dc.contributor.advisor Edward S. Yeung
dc.contributor.author Hsin, Tse-Ming
dc.contributor.department Chemistry
dc.date 2018-08-22T22:37:13.000
dc.date.accessioned 2020-06-30T07:45:13Z
dc.date.available 2020-06-30T07:45:13Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2007
dc.date.issued 2007-01-01
dc.description.abstract <p>Four different laser-based techniques were applied to study physical and chemical characteristics of biomolecules and dye molecules. These techniques are hole burning spectroscopy, single molecule spectroscopy, time-resolved coherent anti-Stokes Raman spectroscopy and laser-induced fluorescence microscopy.;Results from hole burning and single molecule spectroscopy suggested that two antenna states (C708 & C714) of photosystem I from cyanobacterium Synechocystis PCC 6803 are connected by effective energy transfer and the corresponding energy transfer time is ∼6 ps. In addition, results from hole burning spectroscopy indicated that the chlorophyll dimer of the C714 state has a large distribution of the dimer geometry.;Direct observation of vibrational peaks and evolution of coumarin 153 in the electronic excited state was demonstrated by using the fs/ps CARS, a variation of time-resolved coherent anti-Stokes Raman spectroscopy. In three different solvents, methanol, acetonitrile, and butanol, a vibration peak related to the stretch of the carbonyl group exhibits different relaxation dynamics.;Laser-induced fluorescence microscopy, along with the biomimetic containers---liposomes, allows the measurement of the enzymatic activity of individual alkaline phosphatase from bovine intestinal mucosa without potential interferences from glass surfaces. The result showed a wide distribution of the enzyme reactivity. Protein structural variation is one of the major reasons that are responsible for this highly heterogeneous behavior.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/15527/
dc.identifier.articleid 16526
dc.identifier.contextkey 7030038
dc.identifier.doi https://doi.org/10.31274/rtd-180813-16744
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/15527
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/69169
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/15527/3259470.PDF|||Fri Jan 14 20:42:35 UTC 2022
dc.subject.disciplines Physical Chemistry
dc.subject.keywords Chemistry;Physical chemistry
dc.title Detecting molecular properties by various laser-based techniques
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication 42864f6e-7a3d-4be3-8b5a-0ae3c3830a11
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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