Identification of a Novel Genomic Island Conferring Resistance to Multiple Aminoglycoside Antibiotics in Campylobacter coli

dc.contributor.author Qin, Shangshang
dc.contributor.author Wang, Yang
dc.contributor.author Zhang, Qijing
dc.contributor.author Zhang, Qijing
dc.contributor.author Chen, Xia
dc.contributor.author Shen, Zhangqi
dc.contributor.author Deng, Fengru
dc.contributor.author Wu, Congming
dc.contributor.author Shen, Jianzhong
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-18T23:26:11.000
dc.date.accessioned 2020-07-07T05:14:41Z
dc.date.available 2020-07-07T05:14:41Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2012
dc.date.issued 2012-10-01
dc.description.abstract <p>Historically, the incidence of gentamicin resistance in Campylobacter has been very low, but recent studies reported a high prevalence of gentamicin-resistant Campylobacter isolated from food-producing animals in China. The reason for the high prevalence was unknown and was addressed in this study. PCR screening identified aminoglycoside resistance genes <em>aphA-3</em> and <em>aphA-7</em> and the <em>aadE–sat4–aphA-3</em> cluster among 41 Campylobacter isolates from broiler chickens. Importantly, a novel genomic island carrying multiple aminoglycoside resistance genes was identified in 26 aminoglycoside resistant Campylobacter coli strains. Sequence analysis revealed that the genomic island was inserted between <em>cadF</em> and <em>COO1582</em> on the C. coli chromosome and consists of 14 open reading frames (ORFs), including 6 genes (the <em>aadE–sat4–aphA-3</em> cluster, <em>aacA-aphD</em>, <em>aac</em>, and <em>aadE</em>) encoding aminoglycoside-modifying enzymes. Analysis by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing indicated that the C. coli isolates carrying this unique genomic island were clonal, and the clone of PFGE subtype III and sequence type (ST) 1625 was particularly predominant among the C. coli isolates examined, suggesting that clonal expansion may be involved in dissemination of this resistance island. Additionally, we were able to transfer this genomic island from C. coli to a Campylobacter jejuni strain using natural transformation under laboratory conditions, and the transfer resulted in a drastic increase in aminoglycoside resistance in the recipient strain. These findings identify a previously undescribed genomic island that confers resistance to multiple aminoglycoside antibiotics. Since aminoglycoside antibiotics are used for treating occasional systemic infections caused by Campylobacter, the emergence and spread of this antibiotic resistance genomic island represent a potential concern for public health.</p>
dc.description.comments <p>This article is published as Qin, Shangshang, Yang Wang, Qijing Zhang, Xia Chen, Zhangqi Shen, Fengru Deng, Congming Wu, and Jianzhong Shen. "Identification of a novel genomic island conferring resistance to multiple aminoglycoside antibiotics in Campylobacter coli." Antimicrobial agents and chemotherapy 56, no. 10 (2012): 5332-5339. doi: <a href="http://dx.doi.org/10.1128/AAC.00809-12%20" target="_blank" title="Identification of a Novel Genomic Island Conferring Resistance to Multiple Aminoglycoside Antibiotics in Campylobacter coli">10.1128/AAC.00809-12</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/161/
dc.identifier.articleid 1160
dc.identifier.contextkey 10824387
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/161
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92267
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/161/2012_Zhang_IdentificationNovel.pdf|||Fri Jan 14 20:55:07 UTC 2022
dc.source.uri 10.1128/AAC.00809-12
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Identification of a Novel Genomic Island Conferring Resistance to Multiple Aminoglycoside Antibiotics in Campylobacter coli
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 1c6a5dfc-c604-457f-85be-122910db782e
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
File
Original bundle
Now showing 1 - 1 of 1
Name:
2012_Zhang_IdentificationNovel.pdf
Size:
686.78 KB
Format:
Adobe Portable Document Format
Description:
Collections