High prevalence of fluoroquinolone-resistant Campylobacter in sheep and increased Campylobacter counts in the bile and gallbladder of sheep medicated with tetracycline in feed

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dc.contributor.author Plummer, Paul
dc.contributor.author Xia, Jing
dc.contributor.author Pang, Jinji
dc.contributor.author Zhang, Qijing
dc.contributor.author Tang, Yizhi
dc.contributor.author Wu, Zuowei
dc.contributor.author Dai, Lei
dc.contributor.author Singh, Kritika
dc.contributor.author Xu, Changyun
dc.contributor.author Ruddell, Brandon
dc.contributor.author Kreuder, Amanda
dc.contributor.author Xia, Lining
dc.contributor.author Ma, Xiaoping
dc.contributor.author Brooks, Kelly
dc.contributor.author Ocal, Melda
dc.contributor.author Sahin, Orhan
dc.contributor.author Plummer, Paul
dc.contributor.author Griffith, Ronald
dc.contributor.author Zhang, Qijing
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date 2019-05-15T03:54:31.000
dc.date.accessioned 2020-07-07T05:15:01Z
dc.date.available 2020-07-07T05:15:01Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2019
dc.date.embargo 2019-09-29
dc.date.issued 2019-01-01
dc.description.abstract <p><em>Campylobacter</em> is a major foodborne pathogen in humans and a significant cause of abortion in sheep. Although ruminants are increasingly recognized as important reservoirs for <em>Campylobacter</em>, limited information is available about the molecular epidemiology and antimicrobial resistance (AMR) profiles of sheep <em>Campylobacter</em>. Here we describe a two-trial study that examined <em>Campylobacter</em> profiles in sheep and determined whether in-feed tetracycline influenced the distribution and AMR profiles of <em>Campylobacter</em>. Each trial involved 80 commercial sheep naturally infected with <em>Campylobacter</em>, 40 of which were medicated with tetracycline in feed, while the other 40 received feed without antibiotics. Fecal and bile samples were collected for the isolation of <em>Campylobacter</em>. The bacterial isolates were analyzed for antimicrobial susceptibility and genotypes. The results revealed that 87.0% and 61.3% of the fecal and bile samples were positive for <em>Campylobacter</em> (<em>C. jejuni</em> and <em>C. coli</em>), with no significant differences between the medicated and non-medicated groups. All but one of the tested <em>Campylobacter</em> isolates were resistant to tetracycline. Although fluoroquinolone (FQ) resistance remained low in <em>C. jejuni</em> (1.7%), 95.0% of the <em>C. coli</em> isolates were resistant to FQ. Genotyping revealed that <em>C. jejuni</em> ST2862 and <em>C. coli</em> ST902 were the predominant genotypes in the sheep. Feed medication with tetracycline did not affect the overall prevalence, species distribution and AMR profiles of <em>Campylobacter</em>, but increased the total <em>Campylobacter</em> counts in bile and gallbladder. These findings identify predominant <em>Campylobacter</em> clones, reveal the high prevalence of FQ-resistant <em>C. coli</em>, and provide new insights into the epidemiology of <em>Campylobacter</em> in sheep.</p>
dc.description.comments <p>This is a manuscript of an article published as Xia, Jing, Jinji Pang, Yizhi Tang, Zuowei Wu, Lei Dai, Kritika Singh, Changyun Xu, Brandon Ruddell, Amanda Kreuder, Lining Xia, Xiaoping Ma, Kelly S. Brooks, Melda M. Ocal, Orhan Sahin, Paul J. Plummer, Ronald W. Griffith, and Qijing Zhang. "High prevalence of fluoroquinolone-resistant Campylobacter in sheep and increased Campylobacter counts in the bile and gallbladder of sheep medicated with tetracycline in feed." <em>Applied and Environmental Microbiology</em> (2019). DOI: <a href="http://dx.doi.org/10.1128/AEM.00008-19" target="_blank">10.1128/AEM.00008-19</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/208/
dc.identifier.articleid 1207
dc.identifier.contextkey 14163963
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/208
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92319
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/208/2019_ZhangQijing_HighPrevalence.pdf|||Fri Jan 14 22:28:49 UTC 2022
dc.source.uri 10.1128/AEM.00008-19
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Veterinary Infectious Diseases
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.keywords Campylobacter
dc.subject.keywords sheep
dc.subject.keywords antimicrobial resistance
dc.subject.keywords genotype
dc.subject.keywords fluoroquinolone
dc.title High prevalence of fluoroquinolone-resistant Campylobacter in sheep and increased Campylobacter counts in the bile and gallbladder of sheep medicated with tetracycline in feed
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication f16d5130-7036-437d-892d-b4a4072903e7
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relation.isOrgUnitOfPublication 5ab07352-4171-4f53-bbd7-ac5d616f7aa8
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