Mapping and Genotypic Analysis of the NK-Lysin Gene in Chicken

dc.contributor.author Lee, Mi
dc.contributor.author Yang, Ence
dc.contributor.author Lamont, Susan
dc.contributor.author Morisson, Mireille
dc.contributor.author Vignal, Alain
dc.contributor.author Huang, Yong-Zhen
dc.contributor.author Cheng, Hans
dc.contributor.author Muir, William
dc.contributor.author Lamont, Susan
dc.contributor.author Lillehoj, Hyun
dc.contributor.author Lee, Sung
dc.contributor.author Womack, James
dc.contributor.department Animal Science
dc.date 2018-02-17T05:56:09.000
dc.date.accessioned 2020-06-29T23:38:49Z
dc.date.available 2020-06-29T23:38:49Z
dc.date.issued 2014-07-01
dc.description.abstract <p>Antimicrobial peptides (AMP) are important elements of the first line of defence against pathogens in animals. NK-lysin is a cationic AMP that plays a critical role in innate immunity. The chicken <em>NK-lysin</em> gene has been cloned and its antimicrobial and anticancer activity has been described but its location in the chicken genome remains unknown. Here, we mapped the <em>NK-lysin</em> gene and examined the distribution of a functionally significant single nucleotide polymorphism (SNP) among different chicken inbred lines and heritage breeds. A 6000 rad radiation hybrid panel (ChickRH6) was used to map the <em>NK-lysin</em> gene to the distal end of chromosome 22. Two additional genes, the <em>adipocyte enhancer-binding protein 1-lik</em>e gene (<em>AEBP1</em>) and the <em>DNA polymerase delta subunit 2-like</em> (<em>POLD2</em>) gene, are located in the same NW_003779909 contig as <em>NK-lysin</em>, and were thus indirectly mapped to chromosome 22 as well. Previously, we reported a functionally significant SNP at position 271 of the <em>NK-lysin</em> coding sequence in two different chicken breeds. Here, we examined this SNP and found that the A allele appears to be more common than the G allele in these heritage breeds and inbred lines. The chicken <em>NK-lysin</em> gene mapped to the distal end of chromosome 22. Two additional genes,<em>AEBP1</em> and <em>POLD2</em>, were indirectly mapped to chromosome 22 also. SNP analyses revealed that the A allele, which encodes a peptide with a higher antimicrobial activity, is more common than the G allele in our tested inbred lines and heritage breeds.</p>
dc.description.comments <p>This article is from <em>Genetics Selection Evolution</em> 46 (2014): 43, doi:<a href="http://dx.doi.org/10.1186/1297-9686-46-43" target="_blank">10.1186/1297-9686-46-43</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/185/
dc.identifier.articleid 1181
dc.identifier.contextkey 7842127
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/185
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/9588
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/185/2014_Lamont_MappingGenotypic.pdf|||Fri Jan 14 21:42:56 UTC 2022
dc.source.uri 10.1186/1297-9686-46-43
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Poultry or Avian Science
dc.title Mapping and Genotypic Analysis of the NK-Lysin Gene in Chicken
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 5dee3d24-aa7a-4fe1-abf6-f0bb615bfe24
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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