Studies on soybean tissue culture and transformation

dc.contributor.advisor Clifford E. LaMotte Kim, JooHag
dc.contributor.department Botany 2018-08-16T06:52:01.000 2020-07-02T06:13:33Z 2020-07-02T06:13:33Z Mon Jan 01 00:00:00 UTC 1990 1990
dc.description.abstract <p>Several aspects of soybean (Glycine max (L) Merr.) tissue culture and its transformation were examined in this study. A protocol for regeneration of soybean plants from excised parts of 7-day-old Peking seedlings has been developed. Two kinds of explants were used: excised cotyledons and explants each consisting of cotyledonary and primary leaf nodes, the internode that joins them, and one attached cotyledon. When both explants were cultured in vitro on the highly effective shoot-induction medium devised in this study, 35 and 37 shoots per explant on the average were produced from the petiol of the excised cotyledon and from the primary leaf node, respectively. The shoot-induction medium is a modified Murashige and Skoog (MS) medium containing the normal level of MS inorganic macronutrients, fourfold inorganic micronutrients except cobalt, B5 vitamins, 3 g · 1[superscript]-1 sucrose, 2 g · 1[superscript]-1 L-proline, 2 mg · 1[superscript]-1 N[superscript]6-benzyladenine, and 0.02 mg · 1[superscript]-1 [alpha]-naphthaleneacetic acid. Added proline and the increased inorganic micronutrients interact synergistically on shoot regeneration from both kinds of explant, but increasing the micronutrient supply alone has little or no effect. This latter finding seems to explain why earlier attempts to increase shoot regeneration by raising micronutrient did not succeed;The effect of each element of MS micronutrients on shoot regeneration was tested by either singly omitting or adding each element, or raising or lowering the concentration of each element in the medium. Among the seven elements of the MS micronutrients mixture, zinc is the most limiting and all except cobalt are in less than optimal concentration when tested in the proline-containing medium;Small, apparently transformed shoots have been obtained by using Agrobacterium-mediated gene transfer and the productive regeneration system involving shoot regeneration from primary leaf node explants. Agrobacterium strain A281, LBA4404, and A208 caused shoot transformation judging from kanamycin resistance and [beta]-glucuronidase (GUS) activity.</p>
dc.format.mimetype application/pdf
dc.identifier archive/
dc.identifier.articleid 10447
dc.identifier.contextkey 6359979
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/9448
dc.language.iso en
dc.source.bitstream archive/|||Sat Jan 15 02:33:12 UTC 2022
dc.subject.disciplines Botany
dc.subject.keywords Botany
dc.subject.keywords Physiology and molecular biology
dc.title Studies on soybean tissue culture and transformation
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication dissertation Doctor of Philosophy
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
2.29 MB
Adobe Portable Document Format