Roles of manganese and protein kinase signaling in cell culture and animal models of prion disease

dc.contributor.advisor Anumantha G. Kanthasamy
dc.contributor.author Martin, Dustin
dc.contributor.department Biomedical Sciences
dc.date 2018-08-11T09:49:03.000
dc.date.accessioned 2020-06-30T02:49:21Z
dc.date.available 2020-06-30T02:49:21Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2013
dc.date.embargo 2014-09-01
dc.date.issued 2013-01-01
dc.description.abstract <p>Despite several decades of dedicated research by a diverse array of researchers from around the globe, the molecular mechanisms underlying neuronal loss during transmissible spongiform encephalopathy (TSE) remain to be elucidated. Additionally, the etiology of so-called "sporadic" cases of TSE, which represents the vast majority of cases in both humans and animals, remains unknown. Several recent advancements in the understanding of other protein misfolding neurodegenerative disorders including Alzheimer's Disease (AD), Parkinson's Disease (PD), Amyotrophic Lateral Sclerosi (ALS), and TSE have highlighted the similarities between disease etiopathogenesis which may broaden the diversity of potential future therapies. As our understanding of each disease state increases, the potential of advancements from one disease state applying to another is tantalizingly possible. Conversely, the public health implications of the discovery that AD, PD, and ALS may be transmitted in a prion-like manner highlight the urgency in elucidating the mechanisms underlying these devastating disorders. Although the metal binding capacity of PrP<sup>C</sup> and other amyloidogenic proteins is well documented, the environmental contribution to TSE pathogenesis, including the role of divalent metals such as manganese, is unknown. Herein we report that neuronal cells infected with TSE are less susceptible to Mn-induced toxicity than uninfected cells. Mn treatment also induces cytosolic localization of PrP<sup>C</sup> in cell and animal models, thus providing a mechanism that incorporates inhibition of the protein degradation machinery with metal dyshomeostasis. Additionally, we have discovered proteolytic activation of the delta isoform of protein kinase C (PKCδ) and changes in the phosphorylation of PKCδ at two regulatory sites in multiple brain regions in a mouse-adapted scrapie model. Knockout of PKCδ causes a significant delay in the onset of motor symptoms associated with TSE and an altered pattern of oxidative damage. These discoveries provide further mechanistic insight into TSE-related neuronal degeneration.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/13388/
dc.identifier.articleid 4395
dc.identifier.contextkey 4615892
dc.identifier.doi https://doi.org/10.31274/etd-180810-721
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/13388
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/27575
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/13388/Martin_iastate_0097E_13738.pdf|||Fri Jan 14 19:51:12 UTC 2022
dc.subject.disciplines Neuroscience and Neurobiology
dc.subject.keywords Mahogunin
dc.subject.keywords Manganese
dc.subject.keywords Neurodegeneration
dc.subject.keywords Prion
dc.subject.keywords Protein Kinase C Delta
dc.title Roles of manganese and protein kinase signaling in cell culture and animal models of prion disease
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication 184db3f2-d93f-4571-8ad7-07c8a9e6a5c9
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
File
Original bundle
Now showing 1 - 1 of 1
Name:
Martin_iastate_0097E_13738.pdf
Size:
6.11 MB
Format:
Adobe Portable Document Format
Description: