Evaluation of a new chromogenic Salmonella plating medium

Date
2001-01-01
Authors
Reissbrodt, Rolf
Gaull, Florian
Fehlhaber, Karsten
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Abstract

Oxoid Salmonella Chromogenic Medium (OSCM) was tested for its sensitivity and specificity as well as its use in isolation of Salmonella from stool specimens and from porcine faeces. Colonies of Salmonella enterica ssp. I, II, IV, and VI-strains grow onto the ivory-coloured turbid plates purple to mauve, size 1-2 mm in diameter. Contrarily, E. coli, Enterobacter spp., Klebsiella spp., S. sonnei and the most of C. freundii strains appear as turquoise to blue colonies. Proteus spp., Providencia spp. and Morganella morganii grow light with brownish precipitation. S. dysenteriae, S. jlexneri and S. boydii are widely inhibited. Pseudomonads, Aeromonas spp., grampositive bacteria and C. albicans are completely inhibited. Its recovery rate and sensitivity in isolation of salmonella from stool specimens is comparable to Hektoen Enteric agar. OSCM was superior in isolation of Salmonella from porcine faeces compared with XLD.

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