Evaluation of a new chromogenic Salmonella plating medium
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The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.
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Abstract
Oxoid Salmonella Chromogenic Medium (OSCM) was tested for its sensitivity and specificity as well as its use in isolation of Salmonella from stool specimens and from porcine faeces. Colonies of Salmonella enterica ssp. I, II, IV, and VI-strains grow onto the ivory-coloured turbid plates purple to mauve, size 1-2 mm in diameter. Contrarily, E. coli, Enterobacter spp., Klebsiella spp., S. sonnei and the most of C. freundii strains appear as turquoise to blue colonies. Proteus spp., Providencia spp. and Morganella morganii grow light with brownish precipitation. S. dysenteriae, S. jlexneri and S. boydii are widely inhibited. Pseudomonads, Aeromonas spp., grampositive bacteria and C. albicans are completely inhibited. Its recovery rate and sensitivity in isolation of salmonella from stool specimens is comparable to Hektoen Enteric agar. OSCM was superior in isolation of Salmonella from porcine faeces compared with XLD.