Gene expression in tonsils in swine following infection with porcine reproductive and respiratory syndrome virus

dc.contributor.author Dong, Qian
dc.contributor.author Lunney, Joan
dc.contributor.author Lim, Kyu-Sang
dc.contributor.author Nguyen, Yet
dc.contributor.author Hess, Andrew
dc.contributor.author Beiki, Hamid
dc.contributor.author Rowland, Raymond
dc.contributor.author Walker, Kristen
dc.contributor.author Reecy, James
dc.contributor.author Tuggle, Christopher
dc.contributor.author Dekkers, Jack
dc.contributor.department Animal Science
dc.contributor.department Statistics
dc.date 2021-02-26T16:48:34.000
dc.date.accessioned 2021-04-29T23:52:15Z
dc.date.available 2021-04-29T23:52:15Z
dc.date.issued 2021-02-22
dc.description.abstract <p>Background: Porcine reproductive and respiratory syndrome (PRRS) is a threat to pig production worldwide. Our objective was to understand mechanisms of persistence of PRRS virus (PRRSV) in tonsil. Transcriptome data from tonsil samples collected at 42 days post infection (dpi) were generated by RNA-seq and NanoString on 51 pigs that were selected to contrast the two PRRSV isolates used, NVSL and KS06, high and low tonsil viral level at 42 dpi, and the favorable and unfavorable genotypes at a genetic marker (WUR) for the putative PRRSV resistance gene GBP5.</p> <p>Results: The number of differentially expressed genes (DEGs) differed markedly between models with and without accounting for cell-type enrichments (CE) in the samples that were predicted from the RNA-seq data. This indicates that differences in cell composition in tissues that consist of multiple cell types, such as tonsil, can have a large impact on observed differences in gene expression. Based on both the NanoString and the RNA-seq data, KS06- infected pigs showed greater activation, or less inhibition, of immune response in tonsils at 42 dpi than NVSLinfected pigs, with and without accounting for CE. This suggests that the NVSL virus may be better than the KS06 virus at evading host immune response and persists in tonsils by weakening, or preventing, host immune responses. Pigs with high viral levels showed larger CE of immune cells than low viral level pigs, potentially to trigger stronger immune responses. Presence of high tonsil virus was associated with a stronger immune response, especially innate immune response through interferon signaling, but these differences were not significant when accounting for CE. Genotype at WUR was associated with different effects on immune response in tonsils of pigs during the persistence stage, depending on viral isolate and tonsil viral level.</p> <p>Conclusions: Results of this study provide insights into the effects of PRRSV isolate, tonsil viral level, and WUR genotype on host immune response and into potential mechanisms of PRRSV persistence in tonsils that could be targeted to improve strategies to reduce viral rebreaks. Finally, to understand transcriptome responses in tissues that consist of multiple cell types, it is important to consider differences in cell composition.</p>
dc.description.comments <p>This article is published as Dong, Q., Lunney, J.K., Lim, KS. <em>et al.</em> Gene expression in tonsils in swine following infection with porcine reproductive and respiratory syndrome virus. <em>BMC Vet Res</em> 17, 88 (2021). doi:<a href="https://doi.org/10.1186/s12917-021-02785-1">10.1186/s12917-021-02785-1</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/789/
dc.identifier.articleid 1790
dc.identifier.contextkey 21843149
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/789
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/104543
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/789/2021_Reecy_GeneExpression.pdf|||Sat Jan 15 01:55:20 UTC 2022
dc.source.uri 10.1186/s12917-021-02785-1
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Immunology and Infectious Disease
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Statistical Models
dc.subject.keywords PRRSV
dc.subject.keywords Persistence
dc.subject.keywords Tonsils
dc.subject.keywords Pig
dc.subject.keywords Isolate
dc.subject.keywords WUR
dc.subject.keywords RNA-seq
dc.subject.keywords Cell composition
dc.title Gene expression in tonsils in swine following infection with porcine reproductive and respiratory syndrome virus
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication 264904d9-9e66-4169-8e11-034e537ddbca
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