A Comparative Analysis of Methylome Profiles of Campylobacter jejuni Sheep Abortion Isolate and Gastroenteric Strains Using PacBio Data

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2015-01-01
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Mou, Kathy
Muppirala, Usha
Plummer, Paul
Severin, Andrew
Clark, Tyson
Boitano, Matthew
Plummer, Paul
Severin, Andrew
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Abstract

Campylobacter jejuni is a leading cause of human gastrointestinal disease and small ruminant abortions in the United States. The recent emergence of a highly virulent, tetracycline-resistant C. jejuni subsp. jejunisheep abortion clone (clone SA) in the United States, and that strain's association with human disease, has resulted in a heightened awareness of the zoonotic potential of this organism. Pacific Biosciences' Single Molecule, Real-Time sequencing technology was used to explore the variation in the genome-wide methylation patterns of the abortifacient clone SA (IA3902) and phenotypically distinct gastrointestinal-specific C. jejuni strains (NCTC 11168 and 81-176). Several notable differences were discovered that distinguished the methylome of IA3902 from that of 11168 and 81-176: identification of motifs novel to IA3902, genome-specific hypo- and hypermethylated regions, strain level variability in genes methylated, and differences in the types of methylation motifs present in each strain. These observations suggest a possible role of methylation in the contrasting disease presentations of these three C. jejuni strains. In addition, the methylation profiles between IA3902 and a luxS mutant were explored to determine if variations in methylation patterns could be identified that might explain the role of LuxS-dependent methyl recycling in IA3902 abortifacient potential.

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<p>This article is from <em>Frontiers in Microbiology</em> 5 (2015): 782, doi:<a href="http://dx.doi.org/10.3389/fmicb.2014.00782" target="_blank">10.3389/fmicb.2014.00782</a>. Posted with permission.</p>
Keywords
methylation, Campylobacter jejuni, LuxS, quorum sensing, methylome
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