A Comparative Analysis of Methylome Profiles of Campylobacter jejuni Sheep Abortion Isolate and Gastroenteric Strains Using PacBio Data

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dc.contributor.author Plummer, Paul
dc.contributor.author Mou, Kathy
dc.contributor.author Muppirala, Usha
dc.contributor.author Severin, Andrew
dc.contributor.author Clark, Tyson
dc.contributor.author Boitano, Matthew
dc.contributor.author Plummer, Paul
dc.contributor.author Severin, Andrew
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.contributor.department Genome Informatics Facility
dc.date 2018-02-17T10:07:44.000
dc.date.accessioned 2020-07-07T05:13:24Z
dc.date.available 2020-07-07T05:13:24Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 2015
dc.date.issued 2015-01-01
dc.description.abstract <p><em>Campylobacter jejuni</em> is a leading cause of human gastrointestinal disease and small ruminant abortions in the United States. The recent emergence of a highly virulent, tetracycline-resistant <em>C. jejuni</em> subsp. <em>jejuni</em>sheep abortion clone (clone SA) in the United States, and that strain's association with human disease, has resulted in a heightened awareness of the zoonotic potential of this organism. Pacific Biosciences' Single Molecule, Real-Time sequencing technology was used to explore the variation in the genome-wide methylation patterns of the abortifacient clone SA (IA3902) and phenotypically distinct gastrointestinal-specific <em>C. jejuni</em> strains (NCTC 11168 and 81-176). Several notable differences were discovered that distinguished the methylome of IA3902 from that of 11168 and 81-176: identification of motifs novel to IA3902, genome-specific hypo- and hypermethylated regions, strain level variability in genes methylated, and differences in the types of methylation motifs present in each strain. These observations suggest a possible role of methylation in the contrasting disease presentations of these three <em>C. jejuni</em> strains. In addition, the methylation profiles between IA3902 and a <em>luxS</em> mutant were explored to determine if variations in methylation patterns could be identified that might explain the role of LuxS-dependent methyl recycling in IA3902 abortifacient potential.</p>
dc.description.comments <p>This article is from <em>Frontiers in Microbiology</em> 5 (2015): 782, doi:<a href="http://dx.doi.org/10.3389/fmicb.2014.00782" target="_blank">10.3389/fmicb.2014.00782</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vdpam_pubs/44/
dc.identifier.articleid 1033
dc.identifier.contextkey 7987719
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vdpam_pubs/44
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92071
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vdpam_pubs/44/2015_Plummer_ComparativeAnalysis.pdf|||Sat Jan 15 00:17:34 UTC 2022
dc.source.uri 10.3389/fmicb.2014.00782
dc.subject.disciplines Genomics
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Veterinary Infectious Diseases
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.keywords methylation
dc.subject.keywords Campylobacter jejuni
dc.subject.keywords LuxS
dc.subject.keywords quorum sensing
dc.subject.keywords methylome
dc.title A Comparative Analysis of Methylome Profiles of Campylobacter jejuni Sheep Abortion Isolate and Gastroenteric Strains Using PacBio Data
dc.type article
dc.type.genre article
dspace.entity.type Publication
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