Enrichment of Echinacea angustifolia with Bauer Alkylamide 11 and Bauer Ketone 23 Increased Anti-inflammatory Potential through Interference with COX-2 Enzyme Activity

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dc.contributor.author Nikolau, Basil
dc.contributor.author Birt, Diane
dc.contributor.author LaLone, Carlie
dc.contributor.author Huang, Nan
dc.contributor.author Rizshsky, Ludmila
dc.contributor.author Yum, Man-Yu
dc.contributor.author Wurtele, Eve
dc.contributor.author Singh, Navrozedeep
dc.contributor.author Hauck, Cathy
dc.contributor.author Nikolau, Basil
dc.contributor.author Wurtele, Eve
dc.contributor.author Kohut, Marian
dc.contributor.author Murphy, Patricia
dc.contributor.author Birt, Diane
dc.contributor.department Food Science and Human Nutrition
dc.date 2018-02-13T04:51:51.000
dc.date.accessioned 2020-06-30T03:59:22Z
dc.date.available 2020-06-30T03:59:22Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2010
dc.date.embargo 2013-11-22
dc.date.issued 2010-01-01
dc.description.abstract <p>Bauer alkylamide 11 and Bauer ketone 23 were previously found to be partially responsible for<em>Echinacea angustifolia</em> anti-inflammatory properties. This study further tested their importance using the inhibition of prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) and nitric oxide (NO) production by RAW264.7 mouse macrophages in the absence and presence of lipopolysaccharide (LPS) and <em>E. angustifolia</em> extracts, phytochemical enriched fractions, or pure synthesized standards. Molecular targets were probed using microarray, qRT-PCR, Western blot, and enzyme assays. Fractions with these phytochemicals were more potent inhibitors of LPS-induced PGE<sub>2</sub> production than <em>E. angustifolia</em> extracts. Microarray did not detect changes in transcripts with phytochemical treatments; however, qRT-PCR showed a decrease in TNF-α and an increase of iNOS transcripts. LPS-induced COX-2 protein was increased by an <em>E. angustifolia</em> fraction containing Bauer ketone 23 and by pure phytochemical. COX-2 activity was decreased with all treatments. The phytochemical inhibition of PGE<sub>2</sub> production by<em>Echinacea</em> may be due to the direct targeting of COX-2 enzyme.</p>
dc.description.comments <p>Posted with permission from <em>Journal of Agricultural and Food Chemistry </em>58, no. 15 (2010): 8573–8584, doi:<a href="http://dx.doi.org/10.1021/jf1014268" target="_blank">10.1021/jf1014268</a>. Copyright 2010 American Chemical Society.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/fshn_ag_pubs/23/
dc.identifier.articleid 1023
dc.identifier.contextkey 3582129
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath fshn_ag_pubs/23
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/37527
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/fshn_ag_pubs/23/Birt_2010_EnrichmentEchinaceaangustifolia.pdf|||Fri Jan 14 22:45:16 UTC 2022
dc.source.uri 10.1021/jf1014268
dc.subject.disciplines Biochemistry
dc.subject.disciplines Food Science
dc.subject.disciplines Genetics
dc.subject.disciplines Human and Clinical Nutrition
dc.subject.disciplines Kinesiology
dc.subject.disciplines Statistics and Probability
dc.subject.keywords Echinacea angustifolia
dc.subject.keywords prostaglandin E2
dc.subject.keywords nitric oxide
dc.subject.keywords Bauer alkylamides
dc.subject.keywords Bauer ketones
dc.subject.keywords anti-inflammatory
dc.subject.keywords fractionation
dc.title Enrichment of Echinacea angustifolia with Bauer Alkylamide 11 and Bauer Ketone 23 Increased Anti-inflammatory Potential through Interference with COX-2 Enzyme Activity
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 73b33082-7300-4c56-8019-d5e13b9898de
relation.isAuthorOfPublication a56e515d-7fd9-4768-9f73-42a248e128b7
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relation.isOrgUnitOfPublication 4b6428c6-1fda-4a40-b375-456d49d2fb80
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