In vitro studies on germination of immature ovules and plant regeneration from cotyledons of Impatiens platypetala Lindl

dc.contributor.advisor Loren C. Stephens
dc.contributor.author Han, Kyungchul
dc.contributor.department Horticulture
dc.date 2018-08-16T12:43:13.000
dc.date.accessioned 2020-07-02T06:14:02Z
dc.date.available 2020-07-02T06:14:02Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 1991
dc.date.issued 1991
dc.description.abstract <p>Two important aspects of in vitro studies on germination and plant regeneration of Impatiens platypetala Lindl. were investigated. In the first study, factors affecting in vitro germination of immature ovules were investigated. In the second study, factors affecting in vitro plant regeneration from cotyledonary explants were investigated;Several carbohydrates inhibited in vitro germination of immature ovules. Germination was inhibited by sucrose or mannitol at 0.05 M, whereas inhibition did not occur until the concentration of glucose was raised to 0.1 M. The percent of germination increased with increasing explant age from 12 to 16 days old. Abnormal seedlings developed at total nitrogen concentrations higher and lower than 30 mM;Organogenic tissues from cotyledonary explants were successfully induced on organogenic tissue induction (OTI) medium. Both NAA and BA were essential for OTI. Glutamine at 500 mg/liter enhanced the OTI response. OTI was also affected by explant type and age. An average of 7.6 shoots per explant were regenerated on shoot induction medium, and shoots were successfully rooted in a basal MS medium containing 30 g/liter sucrose.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/9528/
dc.identifier.articleid 10527
dc.identifier.contextkey 6360127
dc.identifier.doi https://doi.org/10.31274/rtd-180813-9218
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/9528
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/82636
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/9528/r_9126196.pdf|||Sat Jan 15 02:34:25 UTC 2022
dc.subject.disciplines Botany
dc.subject.disciplines Plant Sciences
dc.subject.keywords Horticulture
dc.title In vitro studies on germination of immature ovules and plant regeneration from cotyledons of Impatiens platypetala Lindl
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication df043cd4-424c-49f5-8685-318972aae642
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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