MALDI-MS analysis and imaging of smallmolecule metabolites with 1,5-diaminonaphthalene (DAN)

Date
2014-05-01
Authors
Korte, Andrew
Lee, Young-Jin
Lee, Young Jin
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Altmetrics
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Research Projects
Organizational Units
Ames Laboratory
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Chemistry
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Abstract

1,5-diaminonaphthalene (DAN) has previously been reported as an effective matrix for matrix-assisted laser desorption ioni- zation-mass spectrometry of phospholipids. In the current work, we investigate the use of DAN as a matrix for small metab- olite analysis in negative ion mode. DAN was found to provide superior ionization to the compared matrices for MW < ~400 Da; however, 9-aminoacridine (9-AA) was found to be superior for a uridine diphosphate standard (MW 566 Da). DAN was also found to provide a more representative profile of a natural phospholipid mixture than 9-AA. Finally, DAN and 9-AA were applied for imaging of metabolites directly from corn leaf sections. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.

Description
<p>This article is from <em>Journal of Mass Spectrometry</em> 49 (2014): 737, doi: <a href="http://dx.doi.org/10.1002/jms.3400" target="_blank">10.1002/jms.3400 </a>. Posted with permission.</p>
Keywords
MALDI, mass spectrometry imaging, matrix, metabolites, small molecules, 1, 5-diaminonaphthalene
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