A nematode effector protein similar to annexins in host plants

dc.contributor.author Patel, Nrupali
dc.contributor.author Hamamouch, Noureddine
dc.contributor.author Baum, Thomas
dc.contributor.author Li, Chunying
dc.contributor.author Hewezi, Tarek
dc.contributor.author Hussey, Richard
dc.contributor.author Baum, Thomas
dc.contributor.author Mitchum, Melissa
dc.contributor.author Davis, Eric
dc.contributor.department Plant Pathology and Microbiology
dc.date 2018-02-18T18:44:02.000
dc.date.accessioned 2020-06-30T06:22:19Z
dc.date.available 2020-06-30T06:22:19Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 2009
dc.date.issued 2010-01-01
dc.description.abstract <p>Nematode parasitism genes encode secreted effector proteins that play a role in host infection. A homologue of the expressed <em>Hg4F01</em> gene of the root-parasitic soybean cyst nematode, <em>Heterodera glycines</em>, encoding an annexin-like effector, was isolated in the related <em>Heterodera schachtii</em> to facilitate use of <em>Arabidopsis thaliana</em> as a model host. <em>Hs4F01</em> and its protein product were exclusively expressed within the dorsal oesophageal gland secretory cell in the parasitic stages of <em>H. schachtii.</em> Hs4F01 had a 41% predicted amino acid sequence identity to the nex-1 annexin of <em>C. elegans</em> and 33% identity to annexin-1 (<em>annAt1</em>) of <em>Arabidopsis</em>, it contained four conserved domains typical of the annexin family of calcium and phospholipid binding proteins, and it had a predicted signal peptide for secretion that was present in nematode annexins of only <em>Heterodera</em> spp. Constitutive expression of <em>Hs4F01</em> in wild-type <em>Arabidopsis</em> promoted hyper-susceptibility to <em>H. schachtii</em> infection. Complementation of an <em>AnnAt1</em> mutant by constitutive expression of <em>Hs4F01</em>reverted mutant sensitivity to 75mM NaCl, suggesting a similar function of the Hs4F01 annexin-like effector in the stress response by plant cells. Yeast two-hybrid assays confirmed a specific interaction between Hs4F01 and an <em>Arabidopsis</em> oxidoreductase member of the 2OG-Fe(II) oxygenase family, a type of plant enzyme demonstrated to promote susceptibility to oomycete pathogens. RNA interference assays that expressed double-stranded RNA complementary to <em>Hs4F01</em> in transgenic <em>Arabidopsis</em> specifically decreased parasitic nematode <em>Hs4F01</em> transcript levels and significantly reduced nematode infection levels. The combined data suggest that nematode secretion of an Hs4F01 annexin-like effector into host root cells may mimic plant annexin function during the parasitic interaction.</p>
dc.description.comments <p>This article is published as Patel, Nrupali, Noureddine Hamamouch, Chunying Li, Tarek Hewezi, Richard S. Hussey, Thomas J. Baum, Melissa G. Mitchum, and Eric L. Davis. "A nematode effector protein similar to annexins in host plants." <em>Journal of Experimental Botany</em> 61, no. 1 (2009): 235-248, doi: <a href="https://doi.org/10.1093/jxb/erp293">10.1093/jxb/erp293</a>. Posted with permission.</p>
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dc.identifier archive/lib.dr.iastate.edu/plantpath_pubs/134/
dc.identifier.articleid 1150
dc.identifier.contextkey 10533062
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath plantpath_pubs/134
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/57577
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/plantpath_pubs/134/2010_Baum_NematodeEffector.pdf|||Fri Jan 14 19:51:49 UTC 2022
dc.source.uri 10.1093/jxb/erp293
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Plant Pathology
dc.subject.keywords Arabidopsis thaliana
dc.subject.keywords cyst nematode
dc.subject.keywords Heterodera
dc.subject.keywords parasitism gene
dc.subject.keywords RNAi
dc.subject.keywords secretions
dc.title A nematode effector protein similar to annexins in host plants
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication ea8d53ba-1a8f-4f5c-9b4a-1ecbd67e2f43
relation.isOrgUnitOfPublication a26b5928-54bb-4a0b-a973-95d649d1ad83
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