Biochemical characterization of new P450s in rice diterpenoid metabolism

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2016-04-01
Authors
Brown, Benjamin
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Abstract

The Oryza sativa P450 CYP76M subfamily hydroxylate a range of labdane-related diterpenes (LRD). Previously the CYP76M5-8 enzymes were screened against the range of LRDs found in rice using our metabolic engineering system in Escherichia coli (E. coli) to determine their functions. It was found that, even though these P450s all have very similar amino acid sequences, individual enzymes in this family have different specificities e.g., CYP76M8 is a more promiscuous enzyme, hydroxylating a wider range of LRDs than CYP76M7. This study focuses on screening two more recently discovered P450s in this same family, CYP76M14 & 17. CYP76M14 was found to have a high specificity for a few LRD, whereas CYP76M17 was more promiscuous. This poster will present the data comparing the functions of these six enzymes.

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