Genetic variation in native populations of the laurel wilt pathogen, Raffaelea lauricola, in Taiwan and Japan and the introduced population in the USA

dc.contributor.advisor Caroline E. Wuest
dc.contributor.author Wuest, Caroline
dc.contributor.department Plant Pathology and Microbiology
dc.date 2018-08-11T10:35:08.000
dc.date.accessioned 2020-06-30T03:08:16Z
dc.date.available 2020-06-30T03:08:16Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2016
dc.date.embargo 2017-02-02
dc.date.issued 2016-01-01
dc.description.abstract <p>Laurel wilt is a true vascular wilt disease caused by Raffaelea lauricola, which is a mycangial symbiont of Xyleborus glabratus, an ambrosia beetle. The fungus and vector are both native to Asia, but it is believed that both were introduced to the Savannah, Georgia area about 15 years ago. Laurel wilt has caused widespread mortality on redbay (Persea borbonia) and other members of the Lauraceae in the southeastern USA, and both pathogen and vector have spread as far as Texas. It is thought that there was a single introduction of R. lauricola to the USA, but there are no extensive studies on the genetic variation of R. lauricola populations that would suggest a genetic bottleneck in the USA. Ten isolates of R. lauricola from Japan, 55 from Taiwan, and 125 from the USA that were collected from X. glabratus adults or infected trees were analyzed with microsatellite and 28S rDNA markers, and with primers developed for two mating type genes. The new primers identified isolates as either MAT1 or MAT2 mating types in roughly equal proportions in Taiwan and Japan, where there was also high genetic diversity within populations based on all the markers, indicating these that individuals within these populations may have cryptic sex. Aside from a local population near Savannah and a single isolate in Alabama that had unique microsatellite alleles, the USA population was genetically uniform and included only the MAT2 mating type, indicating that the population in the USA has undergone a severe genetic bottleneck. This study suggests the importance of preventing a second introduction of R. lauricola to the USA, which could introduce the opposite mating type and allow for genetic recombination and a more aggressive strain of R. lauricola.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/16039/
dc.identifier.articleid 7046
dc.identifier.contextkey 11169598
dc.identifier.doi https://doi.org/10.31274/etd-180810-5666
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/16039
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/30222
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/16039/Wuest_iastate_0097M_16012.pdf|||Fri Jan 14 20:54:14 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Plant Pathology
dc.subject.keywords genetic variation
dc.subject.keywords laurel wilt
dc.subject.keywords Raffaelea lauricola
dc.title Genetic variation in native populations of the laurel wilt pathogen, Raffaelea lauricola, in Taiwan and Japan and the introduced population in the USA
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
relation.isOrgUnitOfPublication a26b5928-54bb-4a0b-a973-95d649d1ad83
thesis.degree.discipline Plant Pathology
thesis.degree.level thesis
thesis.degree.name Master of Science
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