Dynamic transcriptome profiling of Bean Common Mosaic Virus (BCMV) infection in Common Bean (Phaseolus vulgaris L.)

dc.contributor.author Martin, Kathleen
dc.contributor.author Singh, Jugpreet
dc.contributor.author Hill, John
dc.contributor.author Whitham, Steven
dc.contributor.author Whitham, Steven
dc.contributor.author Cannon, Steven
dc.contributor.department Plant Pathology and Microbiology
dc.contributor.department Agronomy
dc.date 2018-02-19T07:09:15.000
dc.date.accessioned 2020-06-30T06:22:53Z
dc.date.available 2020-06-30T06:22:53Z
dc.date.issued 2016-01-01
dc.description.abstract <p><h3>Background</h3> <p id="x-x-x-Par1"><em>Bean common mosaic virus</em> (BCMV) is widespread, with <em>Phaseolus</em> species as the primary host plants. Numerous BCMV strains have been identified on the basis of a panel of bean varieties that distinguish the pathogenicity types with respect to the viral strains. The molecular responses in <em>Phaseolus</em> to BCMV infection have not yet been well characterized. <h3>Results</h3> <p id="x-x-x-Par2">We report the transcriptional responses of a widely susceptible variety of common bean (<em>Phaseolus vulgaris</em> L., cultivar ‘Stringless green refugee’) to two BCMV strains, in a time-course experiment. We also report the genome sequence of a previously unreported BCMV strain. The interaction with the known strain NL1-Iowa causes moderate symptoms and large transcriptional responses, and the newly identified strain (Strain 2 or S2) causes severe symptoms and moderate transcriptional responses. The transcriptional profiles of host plants infected with the two isolates are distinct, and involve numerous differences in splice forms in particular genes, and pathway specific expression patterns. <h3>Conclusions</h3> <p id="x-x-x-Par3">We identified differential host transcriptome response after infection of two different strains of Bean common mosaic virus (BCMV) in common bean (<em>Phaseolus vulgaris</em> L.). Virus infection initiated a suite of changes in gene expression level and patterns in the host plants. Pathways related to defense, gene regulation, metabolic processes, photosynthesis were specifically altered after virus infection. Results presented in this study can increase the understanding of host-pathogen interactions and provide resources for further investigations of the biological mechanisms in BCMV infection and defense.</p>
dc.description.comments <p>This article is published as Martin, Kathleen, Jugpreet Singh, John H. Hill, Steven A. Whitham, and Steven B. Cannon. "Dynamic transcriptome profiling of Bean Common Mosaic Virus (BCMV) infection in Common Bean (Phaseolus vulgaris L.)." BMC genomics 17, no. 1 (2016): 613. doi: <a href="https://doi.org/10.1186/s12864-016-2976-8" target="_blank">10.1186/s12864-016-2976-8</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/plantpath_pubs/208/
dc.identifier.articleid 1207
dc.identifier.contextkey 11340635
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath plantpath_pubs/208
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/57655
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/plantpath_pubs/208/2016_Whitham_DynamicTranscriptome.pdf|||Fri Jan 14 22:28:44 UTC 2022
dc.source.uri 10.1186/s12864-016-2976-8
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.disciplines Plant Pathology
dc.subject.keywords Gene expression
dc.subject.keywords Gene regulation
dc.subject.keywords Host-virus interaction
dc.subject.keywords Regulatory changes
dc.subject.keywords Phaseolus vulgaris
dc.subject.keywords Bean common mosaic virus
dc.title Dynamic transcriptome profiling of Bean Common Mosaic Virus (BCMV) infection in Common Bean (Phaseolus vulgaris L.)
dc.type article
dc.type.genre article
dspace.entity.type Publication
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