Single drop microextraction in a 96-well plate format: A step toward automated and high-throughput analysis

Mafra, Gabriela
Vieira, Augusto
Merib, Josias
Anderson, Jared
Carasek, Eduardo
Journal Title
Journal ISSN
Volume Title
Research Projects
Organizational Units
Organizational Unit
Journal Issue

In this study, an innovative and high-throughput parallel-single-drop microextraction (Pa-SDME) using the [P6,6,6,14+]2[MnCl42−] magnetic ionic liquid (MIL) as extraction phase is demonstrated, for the first time, in the determination of methylparaben, ethylparaben, propylparaben, bisphenol A, butylparaben, benzophenone and triclocarban from environmental aqueous samples. This experimental setup comprised of a 96-well plate system containing a set of magnetic pins which aided in stabilizing the MIL drops and enabled the simultaneous extraction of up to 96 samples. Using this low-cost experimental apparatus, the sample throughput was lower than 1 min per sample. This novel approach exhibits a number of advantages over classical SDME approaches, particularly in maintaining a stable solvent microdrop and facilitating high-throughput analysis. Experimental conditions were carefully optimized using one-factor-at-a-time and multivariate designs. The optimal conditions employed 5.38 ± 0.55 mg (n = 10) of MIL, a sample volume of 1.5 mL at pH 6, and dilution in 20 μL of acetonitrile. The analytical parameters of merit were determined under the optimized conditions and highly satisfactory results were achieved, with LODs ranging from 1.5 to 3 μg L−1 and coefficients of determination higher than 0.994. Intraday and interday precision ranged from 0.6 to 21.3% (n = 3) and 10.4–20.2% (n = 9), respectively, with analyte relative recovery in three aqueous samples ranging between 63% and 126%.


This is a manuscript of an article published as Mafra, Gabriela, Augusto A. Vieira, Josias Merib, Jared L. Anderson, and Eduardo Carasek. "Single drop microextraction in a 96-well plate format: a step toward automated and high-throughput analysis." Analytica Chimica Acta (2019). DOI: 10.1016/j.aca.2019.02.013. Posted with permission.

Magnetic ionic liquids, Parallel single drop microextraction, Sample preparation, Endocrine disrupters compounds, High-throughput, 96-well plate