Single drop microextraction in a 96-well plate format: A step toward automated and high-throughput analysis Mafra, Gabriela Vieira, Augusto Anderson, Jared Merib, Josias Anderson, Jared Carasek, Eduardo
dc.contributor.department Chemistry 2019-03-08T20:02:13.000 2020-06-30T01:16:40Z 2020-06-30T01:16:40Z Tue Jan 01 00:00:00 UTC 2019 2020-02-21 2019-01-01
dc.description.abstract <p>In this study, an innovative and high-throughput parallel-single-drop microextraction (Pa-SDME) using the [P6,6,6,14+]2[MnCl42−] magnetic ionic liquid (MIL) as extraction phase is demonstrated, for the first time, in the determination of methylparaben, ethylparaben, propylparaben, bisphenol A, butylparaben, benzophenone and triclocarban from environmental aqueous samples. This experimental setup comprised of a 96-well plate system containing a set of magnetic pins which aided in stabilizing the MIL drops and enabled the simultaneous extraction of up to 96 samples. Using this low-cost experimental apparatus, the sample throughput was lower than 1 min per sample. This novel approach exhibits a number of advantages over classical SDME approaches, particularly in maintaining a stable solvent microdrop and facilitating high-throughput analysis. Experimental conditions were carefully optimized using one-factor-at-a-time and multivariate designs. The optimal conditions employed 5.38 ± 0.55 mg (n = 10) of MIL, a sample volume of 1.5 mL at pH 6, and dilution in 20 μL of acetonitrile. The analytical parameters of merit were determined under the optimized conditions and highly satisfactory results were achieved, with LODs ranging from 1.5 to 3 μg L−1 and coefficients of determination higher than 0.994. Intraday and interday precision ranged from 0.6 to 21.3% (n = 3) and 10.4–20.2% (n = 9), respectively, with analyte relative recovery in three aqueous samples ranging between 63% and 126%.</p>
dc.description.comments <p>This is a manuscript of an article published as Mafra, Gabriela, Augusto A. Vieira, Josias Merib, Jared L. Anderson, and Eduardo Carasek. "Single drop microextraction in a 96-well plate format: a step toward automated and high-throughput analysis." <em>Analytica Chimica Acta</em> (2019). DOI: <a href="" target="_blank">10.1016/j.aca.2019.02.013</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/
dc.identifier.articleid 2110
dc.identifier.contextkey 13935811
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath chem_pubs/1107
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 18:41:31 UTC 2022
dc.source.uri 10.1016/j.aca.2019.02.013
dc.subject.disciplines Analytical Chemistry
dc.subject.keywords Magnetic ionic liquids
dc.subject.keywords Parallel single drop microextraction
dc.subject.keywords Sample preparation
dc.subject.keywords Endocrine disrupters compounds
dc.subject.keywords High-throughput
dc.subject.keywords 96-well plate
dc.title Single drop microextraction in a 96-well plate format: A step toward automated and high-throughput analysis
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 9618e7bf-b6eb-4753-affc-fca4bcc405cb
relation.isOrgUnitOfPublication 42864f6e-7a3d-4be3-8b5a-0ae3c3830a11
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