Microbiota of the Gut-Lymph Node Axis: Depletion of Mucosa-Associated Segmented Filamentous Bacteria and Enrichment of Methanobrevibacter by Colistin Sulfate and Linco-Spectin in Pigs

dc.contributor.author Zwirzitz, Benjamin
dc.contributor.author Pinior, Beate
dc.contributor.author Schmitz-Esser, Stephan
dc.contributor.author Metzler-Zebeli, Barbara
dc.contributor.author Handler, Monika
dc.contributor.author Gense, Kristina
dc.contributor.author Knecht, Christian
dc.contributor.author Ladinig, Andrea
dc.contributor.author Dzieciol, Monika
dc.contributor.author Wetzels, Stefanie
dc.contributor.author Wagner, Martin
dc.contributor.author Schmitz-Esser, Stephan
dc.contributor.author Mann, Evelyne
dc.contributor.department Animal Science
dc.date 2020-01-09T18:34:07.000
dc.date.accessioned 2020-06-29T23:41:30Z
dc.date.available 2020-06-29T23:41:30Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2019
dc.date.issued 2019-04-01
dc.description.abstract <p>Microorganisms are translocated from the gut to lymphatic tissues via immune cells, thereby challenging and training the mammalian immune system. Antibiotics alter the gut microbiome and consecutively might also affect the corresponding translocation processes, resulting in an imbalanced state between the intestinal microbiota and the host. Hence, understanding the variant effects of antibiotics on the microbiome of gut-associated tissues is of vital importance for maintaining metabolic homeostasis and animal health. In the present study, we analyzed the microbiome of (i) pig feces, ileum, and ileocecal lymph nodes under the influence of antibiotics (Linco-Spectin and Colistin sulfate) using 16S rRNA gene sequencing for high-resolution community profiling and (ii) ileocecal lymph nodes in more detail with two additional methodological approaches, i.e., cultivation of ileocecal lymph node samples and (iii) metatranscriptome sequencing of a single lymph node sample. Supplementation of medicated feed showed a local effect on feces and ileal mucosa-associated microbiomes. Pigs that received antibiotics harbored significantly reduced amounts of segmented filamentous bacteria (SFB) along the ileal mucosa (<em>p</em> = 0.048; 199.17-fold change) and increased amounts of <em>Methanobrevibacter</em>, a methanogenic Euryarchaeote in fecal samples (<em>p</em> = 0.005; 20.17-fold change) compared to the control group. Analysis of the porcine ileocecal lymph node microbiome exposed large differences between the viable and the dead fraction of microorganisms and the microbiome was altered to a lesser extent by antibiotics compared with feces and ileum. The core microbiome of lymph nodes was constituted mainly of <em>Proteobacteria</em>. RNA-sequencing of a single lymph node sample unveiled transcripts responsible for amino acid and carbohydrate metabolism as well as protein turnover, DNA replication and signal transduction. The study presented here is the first comparative study of microbial communities in feces, ileum, and its associated ileocecal lymph nodes. In each analyzed site, we identified specific phylotypes susceptible to antibiotic treatment that can have profound impacts on the host physiological and immunological state, or even on global biogeochemical cycles. Our results indicate that pathogenic bacteria, e.g., enteropathogenic <em>Escherichia coli</em>, could escape antibiotic treatment by translocating to lymph nodes. In general ileocecal lymph nodes harbor a more diverse and active community of microorganisms than previously assumed.</p>
dc.description.comments <p>This article is published as Zwirzitz B, Pinior B, Metzler-Zebeli B, Handler M, Gense K, Knecht C, Ladinig A, Dzieciol M, Wetzels SU, Wagner M, Schmitz-Esser S and Mann E (2019) Microbiota of the Gut-Lymph Node Axis: Depletion of Mucosa-Associated Segmented Filamentous Bacteria and Enrichment of Methanobrevibacter by Colistin Sulfate and Linco-Spectin in Pigs. Front. Microbiol. 10:599. doi: <a href="https://doi.org/10.3389/fmicb.2019.00599">10.3389/fmicb.2019.00599</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/521/
dc.identifier.articleid 1513
dc.identifier.contextkey 16138626
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/521
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/9958
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/521/2019_Schmitz_MicrobiotaGut.pdf|||Sat Jan 15 00:47:58 UTC 2022
dc.source.uri 10.3389/fmicb.2019.00599
dc.subject.disciplines Animal Experimentation and Research
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Environmental Microbiology and Microbial Ecology
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.keywords antibiotics
dc.subject.keywords lymph nodes
dc.subject.keywords ileum
dc.subject.keywords microbiome
dc.subject.keywords 16S rRNA gene
dc.subject.keywords metatranscriptome
dc.subject.keywords segmented filamentous bacteria
dc.subject.keywords gut microbiota
dc.title Microbiota of the Gut-Lymph Node Axis: Depletion of Mucosa-Associated Segmented Filamentous Bacteria and Enrichment of Methanobrevibacter by Colistin Sulfate and Linco-Spectin in Pigs
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 247d6138-be53-44be-9e88-220424e98124
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
File
Original bundle
Now showing 1 - 1 of 1
Name:
2019_Schmitz_MicrobiotaGut.pdf
Size:
2.38 MB
Format:
Adobe Portable Document Format
Description:
Collections