Lifelong quercetin enrichment and cardioprotection in Mdx/Utrn+/− mice

dc.contributor.author Ballmann, Christopher
dc.contributor.author Denney, Thomas
dc.contributor.author Selsby, Joshua
dc.contributor.author Beyers, Ronald
dc.contributor.author Quindry, Tiffany
dc.contributor.author Romero, Matthew
dc.contributor.author Amin, Rajesh
dc.contributor.author Selsby, Joshua
dc.contributor.author Quindry, John
dc.contributor.department Animal Science
dc.date 2021-01-28T21:59:19.000
dc.date.accessioned 2021-02-24T21:13:32Z
dc.date.available 2021-02-24T21:13:32Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2017
dc.date.issued 2017-01-01
dc.description.abstract <p>Duchenne Muscular Dystrophy (DMD) is associated with progressive cardiac pathology, however, the SIRT1/PGC1-α activator quercetin may cardioprotect dystrophic hearts. We tested the extent to which long term 0.2% dietary quercetin enrichment attenuates dystrophic cardiopathology in Mdx/Utrn+/- mice. At 2 months, Mdx/Utrn+/- mice were fed quercetin enriched (Mdx/Utrn+/--Q) or control diet (Mdx/Utrn+/-) for 8 months. Control C57BL/10 (C57) animals were fed a control diet for 10 months. Cardiac function was quantified by MRI at 2 and 10 months. Spontaneous physical activity was quantified during the last week of treatment. At 10 months hearts were excised for histological and biochemical analysis. Quercetin feeding improved various physiologic indices of cardiac function in diseased animals. Mdx/Utrn+/--Q also engaged in more high intensity physical activity than controls. Histological analyses of heart tissues revealed higher expression and co-localization of utrophin and α-sarcoglycan. Lower abundance of fibronectin, cardiac damage (Hematoxylin Eosin-Y), and MMP9 were observed in quercetin fed versus control Mdx/Utrn+/- mice. Quercetin evoked higher protein abundance of PGC-1α, cytochrome-c, ETC complexes I-V, citrate synthase, SOD2, and GPX compared to control-fed Mdx/Utrn+/-. Quercetin decreased abundance of inflammatory markers including NFκB, TGF-β1, and F4/80 compared to Mdx/Utrn+/-, however, P-NFκB, P-IKBα, IKBα, CD64 and COX2 were similar between groups. Dietary quercetin enrichment improves cardiac function in aged Mdx/Utrn+/- mice, increases mitochondrial protein content, and dystrophin glycoprotein complex formation. Histological analyses indicate a marked attenuation in pathological cardiac remodeling and indicate that long term quercetin consumption benefits the dystrophic heart.</p>
dc.description.comments <p>This is a manuscript of an article published as Ballmann, Christopher, Thomas S. Denney, Ronald J. Beyers, Tiffany Quindry, Matthew Romero, Rajesh Amin, Joshua T. Selsby, and John C. Quindry. "Lifelong quercetin enrichment and cardioprotection in Mdx/Utrn+/− mice." <em>American Journal of Physiology-Heart and Circulatory Physiology</em> 312, no. 1 (2017): H128-H140. doi: <a href="https://doi.org/10.1152/ajpheart.00552.2016">10.1152/ajpheart.00552.2016</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/686/
dc.identifier.articleid 1702
dc.identifier.contextkey 21309801
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/686
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/93441
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/686/2017_Selsby_LifelongQuercetinManuscript.pdf|||Sat Jan 15 01:29:42 UTC 2022
dc.source.uri 10.1152/ajpheart.00552.2016
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Experimentation and Research
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Cardiology
dc.subject.disciplines Physiology
dc.subject.keywords Polyphenol
dc.subject.keywords Utrophin
dc.subject.keywords Duchenne Muscular Dystrophy
dc.title Lifelong quercetin enrichment and cardioprotection in Mdx/Utrn+/− mice
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 6f883eb1-dd01-4874-8949-fa2eaef5899a
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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