Roles of Three Transporters, CbcXWV, BetT1, and BetT3, in Pseudomonas aeruginosa Choline Uptake for Catabolism

dc.contributor.author Malek, Adel
dc.contributor.author Chen, Chiliang
dc.contributor.author Beattie, Gwyn
dc.contributor.author Wargo, Matthew
dc.contributor.author Beattie, Gwyn
dc.contributor.author Hogan, Deborah
dc.contributor.department Plant Pathology and Microbiology
dc.date 2018-02-17T21:08:12.000
dc.date.accessioned 2020-06-30T06:24:02Z
dc.date.available 2020-06-30T06:24:02Z
dc.date.copyright Sat Jan 01 00:00:00 UTC 2011
dc.date.issued 2011-06-01
dc.description.abstract <p>Pseudomonas aeruginosa uses the quaternary amine choline as a carbon source, osmoprotectant, and macromolecular precursor. The importance of choline in P. aeruginosa physiology is highlighted by the presence of multiple known and putative choline transporters encoded within its genome. This report describes the relative roles of three choline transporters, the ABC transporter CbcXWV and two symporters, BetT1 and BetT3, in P. aeruginosa growth on choline under osmotic conditions that are physiologically relevant to eukaryotic hosts. The increased lag phases exhibited by the Δ<em>betT1</em> and Δ<em>betT1</em> Δ<em>betT3</em> mutants relative to the wild type upon transfer to medium with choline as a sole carbon source suggested roles for BetT1 and BetT3 in cells newly exposed to choline. BetT3 and CbcXWV, but not BetT1, were sufficient to support growth on choline. <em>betT1</em> and <em>betT3</em>expression was regulated by the repressor BetI and choline, whereas <em>cbcXWV</em>expression was induced by the activator GbdR and glycine betaine. The data support a model in which, upon transfer to a choline-based medium, the glycine betaine derived from choline taken up by BetT1 and BetT3 promotes subsequent GbdR-mediated <em>cbcXWV</em> induction. Furthermore, growth data indicated that the relative contributions of each transporter varied under different conditions, as BetT1 and CbcXWV were the primary choline transporters under hypo-osmolar conditions whereas BetT3 was the major choline transporter under hyperosmolar conditions. This work represents the first systematic approach to unravel the mechanisms of choline uptake in P. aeruginosa, which has the most complex bacterial choline uptake systems characterized to date.</p>
dc.description.comments <p>This article is from <em>Journal of Bacteriology</em> 193 (2011): 3033, doi: <a href="http://dx.doi.org/10.1128/JB.00160-11" target="_blank">10.1128/JB.00160-11</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/plantpath_pubs/98/
dc.identifier.articleid 1097
dc.identifier.contextkey 8988698
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath plantpath_pubs/98
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/57814
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/plantpath_pubs/98/2011_Beattie_RolesThree.pdf|||Sat Jan 15 02:37:49 UTC 2022
dc.source.uri 10.1128/JB.00160-11
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Plant Biology
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.disciplines Plant Pathology
dc.title Roles of Three Transporters, CbcXWV, BetT1, and BetT3, in Pseudomonas aeruginosa Choline Uptake for Catabolism
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 3fbe9503-8da0-4141-846b-aa8aa673e2f0
relation.isOrgUnitOfPublication a26b5928-54bb-4a0b-a973-95d649d1ad83
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