Genetic lines respond uniquely within the chicken thymic transcriptome to acute heat stress and low dose lipopolysaccharide

dc.contributor.author Rothschild, Max
dc.contributor.author Monson, Melissa
dc.contributor.author Van Goor, Angelica
dc.contributor.author Persia, Michael
dc.contributor.author Lamont, Susan
dc.contributor.author Rothschild, Max
dc.contributor.author Schmidt, Carl
dc.contributor.author Lamont, Susan
dc.contributor.department Animal Science
dc.date 2019-09-25T22:14:51.000
dc.date.accessioned 2020-06-29T23:41:19Z
dc.date.available 2020-06-29T23:41:19Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2019
dc.date.issued 2019-01-01
dc.description.abstract <p>Exposure to high temperatures is known to impair immune functions and disease resistance of poultry. Characterizing changes in the transcriptome can help identify mechanisms by which immune tissues, such as the thymus, respond to heat stress. In this study, 22-day-old chickens from two genetic lines (a relatively resistant Fayoumi line and a more susceptible broiler line) were exposed to acute heat stress (35 °C) and/or immune simulation with lipopolysaccharide (LPS; 100 µg/kg). Transcriptome responses in the thymus were identified by RNA-sequencing (RNA-seq). Expression of most genes was unaffected by heat and/or LPS in the Fayoumi line, whereas these treatments had more impact in the broiler line. Comparisons between the broiler and Fayoumi transcriptomes identified a large number of significant genes both at homeostasis and in response to treatment. Functional analyses predicted that gene expression changes impact immune responses, apoptosis, cell activation, migration, and adhesion. In broilers, acute heat stress changed thymic expression responses to LPS and could impact thymocyte survival and trafficking, and thereby contribute to the negative effects of high temperatures on immune responses. Identification of these genes and pathways provides a foundation for testing targets to improve disease resistance in heat-stressed chickens.</p>
dc.description.comments <p>This article is published as Monson, Melissa S., Angelica G. Van Goor, Michael E. Persia, Max F. Rothschild, Carl J. Schmidt, and Susan J. Lamont. "Genetic lines respond uniquely within the chicken thymic transcriptome to acute heat stress and low dose lipopolysaccharide." <em>Scientific Reports</em> 9 (2019):13649. doi: <a href="https://doi.org/10.1038/s41598-019-50051-0">10.1038/s41598-019-50051-0</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/499/
dc.identifier.articleid 1500
dc.identifier.contextkey 15421998
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/499
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/9933
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/499/2019_Rothschild_GeneticLines.pdf|||Sat Jan 15 00:31:04 UTC 2022
dc.source.uri 10.1038/s41598-019-50051-0
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Genetics
dc.subject.disciplines Poultry or Avian Science
dc.title Genetic lines respond uniquely within the chicken thymic transcriptome to acute heat stress and low dose lipopolysaccharide
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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