A new multiplexed absorbance detection approach for high-throughput PCR analysis, enzyme assay, peptide mapping and combinatorial homogeneous catalysis screening using 96-capillary array electrophoresis

dc.contributor.advisor Edward S. Yeung
dc.contributor.author Gong, Xiaoyi
dc.contributor.department Chemistry
dc.date 2018-08-23T02:04:45.000
dc.date.accessioned 2020-06-30T07:20:04Z
dc.date.available 2020-06-30T07:20:04Z
dc.date.copyright Sat Jan 01 00:00:00 UTC 2000
dc.date.issued 2000-01-01
dc.description.abstract <p>The purpose of this research is to develop a UV-vis absorbance detection approach for multiplexed capillary electrophoresis, and explore the use of multiplexed capillary electrophoresis with UV-vis absorbance detection as a high-throughput analysis technique for various types of applications;We first introduced a novel absorbance detection approach for 96-capillary array electrophoresis. The approach involved the use of a 1024-pixel linear photodiode array on which a 96-capillary array was imaged by a camera lens. The detection limit for Rhodamine 6G for each capillary in the multiplexed array was about 1.8 x 10--8 M (S/N = 2). The cross talk between adjacent capillaries is less than 0.2%. Uniform separation efficiency and signal to noise ratio were obtain across the capillary array. Simultaneous analysis of 96 samples using capillary zone electrophoresis (CZE) was demonstrated;We further successfully applied the 96-capillary array electrophoresis system with UV-vis absorbance detection for high-throughput HIV diagnosis, genetic typing, enzyme assay, protein analysis by peptide mapping, and combinatorial screening of homogeneous catalytic reactions.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/12322/
dc.identifier.articleid 13321
dc.identifier.contextkey 6770140
dc.identifier.doi https://doi.org/10.31274/rtd-180813-13592
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/12322
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/65678
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/12322/r_9990446.pdf|||Fri Jan 14 19:18:41 UTC 2022
dc.subject.disciplines Analytical Chemistry
dc.subject.keywords Chemistry
dc.subject.keywords Analytical chemistry
dc.title A new multiplexed absorbance detection approach for high-throughput PCR analysis, enzyme assay, peptide mapping and combinatorial homogeneous catalysis screening using 96-capillary array electrophoresis
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication 42864f6e-7a3d-4be3-8b5a-0ae3c3830a11
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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