The Turkey Major Histocompatibility Complex: Identification of Class II Genotypes by Restriction Fragment Length Polymorphism Analysis of Deoxyribonucleic Acid

dc.contributor.author Emara, Marlene
dc.contributor.author Nestor, Karl
dc.contributor.author Lamont, Susan
dc.contributor.author Foster, Douglas
dc.contributor.author Lamont, Susan
dc.contributor.department Animal Science
dc.date 2021-04-15T17:51:49.000
dc.date.accessioned 2021-04-29T23:53:26Z
dc.date.available 2021-04-29T23:53:26Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 1992
dc.date.issued 1992-12-01
dc.description.abstract <p>Using a chicken Class II MHC clone in Northern blot analysis, tissue-specific expression of turkey Class II MHC genes was observed in the embryonic bursa of Fabricius as well as in the adult spleen. In contrast, there was no detectable expression in the embryonic liver, brain, or spleen. Southern blot analysis of <em>Bam</em> HI-digested turkey DNA revealed two restriction fragment length polymorphism (RFLP) patterns that did not deviate significantly from single-gene Mendelian inheritance. Further analysis of <em>Pvu</em> II-digested DNA from 325 turkeys showed four distinct RFLP patterns that segregated within the turkey lines studied. Because the chicken Class II MHC clone hybridized specifically to mRNA in immune-associated tissues, and because it identified polymorphisms among turkeys, the chicken clone is suggested to identify four turkey Class II MHC genotypes. The current study provides good evidence that RFLP analysis of DNA can be used as a means for molecular genotyping at the MHC in turkeys.</p>
dc.description.comments <p>This article is published as Emara, Marlene G., Karl E. Nestor, Douglas N. Foster, and Susan J. Lamont. "The turkey major histocompatibility complex: identification of class II genotypes by restriction fragment length polymorphism analysis of deoxyribonucleic acid."<em> Poultry Scienc</em>e 71, no. 12 (1992): 2083-2089. DOI: <a href="https://doi.org/10.3382/ps.0712083" target="_blank">10.3382/ps.0712083</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/840/
dc.identifier.articleid 1842
dc.identifier.contextkey 22489285
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/840
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/104594
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/840/1992_LamontSusan_TurkeyMajor.pdf|||Sat Jan 15 02:11:15 UTC 2022
dc.source.uri 10.3382/ps.0712083
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Genetics
dc.subject.disciplines Poultry or Avian Science
dc.subject.keywords turkey
dc.subject.keywords major histocompatibility complex
dc.subject.keywords restriction fragment length polymorphism
dc.subject.keywords molecular genotyping
dc.subject.keywords Class II genes
dc.title The Turkey Major Histocompatibility Complex: Identification of Class II Genotypes by Restriction Fragment Length Polymorphism Analysis of Deoxyribonucleic Acid
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 5dee3d24-aa7a-4fe1-abf6-f0bb615bfe24
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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