Stochastic Optical Reconstruction Microscopy Imaging of Microtubule Arrays in Intact Arabidopsis thaliana Seedling

dc.contributor.author Dong, Bin
dc.contributor.author Bassham, Diane
dc.contributor.author Yang, Xiaochen
dc.contributor.author Zhu, Shaobin
dc.contributor.author Bassham, Diane
dc.contributor.author Fang, Ning
dc.contributor.department Ames Laboratory
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Chemistry
dc.contributor.department Plant Sciences Institute
dc.date 2019-05-16T18:35:14.000
dc.date.accessioned 2020-06-30T04:01:55Z
dc.date.available 2020-06-30T04:01:55Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 2015
dc.date.issued 2015-01-01
dc.description.abstract <p>Super-resolution fluorescence microscopy has generated tremendous success in revealing detailed subcellular structures in animal cells. However, its application to plant cell biology remains extremely limited due to numerous technical challenges, including the generally high fluorescence background of plant cells and the presence of the cell wall. In the current study, stochastic optical reconstruction microscopy (STORM) imaging of intact <em>Arabidopsis thaliana</em> seedling roots with a spatial resolution of 20–40 nm was demonstrated. Using the super-resolution images, the spatial organization of cortical microtubules in different parts of a whole Arabidopsis root tip was analyzed quantitatively, and the results show the dramatic differences in the density and spatial organization of cortical microtubules in cells of different differentiation stages or types. The method developed can be applied to plant cell biological processes, including imaging of additional elements of the cytoskeleton, organelle substructure, and membrane domains.</p>
dc.description.comments <p>This article is published as Dong, Bin, Xiaochen Yang, Shaobin Zhu, Diane C. Bassham, and Ning Fang. "Stochastic optical reconstruction microscopy imaging of microtubule arrays in intact Arabidopsis thaliana seedling roots." <em>Scientific reports</em> 5 (2015): 15694. doi: <a href="https://doi.org/10.1038/srep15694">10.1038/srep15694</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/gdcb_las_pubs/211/
dc.identifier.articleid 1215
dc.identifier.contextkey 14164816
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath gdcb_las_pubs/211
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/37885
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/gdcb_las_pubs/211/2015_Bassham_StochasticOptical.pdf|||Fri Jan 14 22:34:52 UTC 2022
dc.source.uri 10.1038/srep15694
dc.subject.disciplines Cell Biology
dc.subject.disciplines Developmental Biology
dc.subject.disciplines Plant Biology
dc.title Stochastic Optical Reconstruction Microscopy Imaging of Microtubule Arrays in Intact Arabidopsis thaliana Seedling
dc.type article
dc.type.genre article
dspace.entity.type Publication
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