Stochastic Optical Reconstruction Microscopy Imaging of Microtubule Arrays in Intact Arabidopsis thaliana Seedling Dong, Bin Bassham, Diane Yang, Xiaochen Zhu, Shaobin Bassham, Diane Fang, Ning
dc.contributor.department Ames Laboratory
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Chemistry
dc.contributor.department Plant Sciences Institute 2019-05-16T18:35:14.000 2020-06-30T04:01:55Z 2020-06-30T04:01:55Z Thu Jan 01 00:00:00 UTC 2015 2015-01-01
dc.description.abstract <p>Super-resolution fluorescence microscopy has generated tremendous success in revealing detailed subcellular structures in animal cells. However, its application to plant cell biology remains extremely limited due to numerous technical challenges, including the generally high fluorescence background of plant cells and the presence of the cell wall. In the current study, stochastic optical reconstruction microscopy (STORM) imaging of intact <em>Arabidopsis thaliana</em> seedling roots with a spatial resolution of 20–40 nm was demonstrated. Using the super-resolution images, the spatial organization of cortical microtubules in different parts of a whole Arabidopsis root tip was analyzed quantitatively, and the results show the dramatic differences in the density and spatial organization of cortical microtubules in cells of different differentiation stages or types. The method developed can be applied to plant cell biological processes, including imaging of additional elements of the cytoskeleton, organelle substructure, and membrane domains.</p>
dc.description.comments <p>This article is published as Dong, Bin, Xiaochen Yang, Shaobin Zhu, Diane C. Bassham, and Ning Fang. "Stochastic optical reconstruction microscopy imaging of microtubule arrays in intact Arabidopsis thaliana seedling roots." <em>Scientific reports</em> 5 (2015): 15694. doi: <a href="">10.1038/srep15694</a>.</p>
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dc.identifier.articleid 1215
dc.identifier.contextkey 14164816
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dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 22:34:52 UTC 2022
dc.source.uri 10.1038/srep15694
dc.subject.disciplines Cell Biology
dc.subject.disciplines Developmental Biology
dc.subject.disciplines Plant Biology
dc.title Stochastic Optical Reconstruction Microscopy Imaging of Microtubule Arrays in Intact Arabidopsis thaliana Seedling
dc.type article
dc.type.genre article
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