A Tetrahymena thermophila G4-DNA Binding Protein with Dihydrolipoamide Dehydrogenase Activity
dc.contributor.author | Kee, Kehkooi | |
dc.contributor.author | Niu, Luming | |
dc.contributor.author | Henderson, Eric | |
dc.contributor.author | Henderson, Eric | |
dc.contributor.department | Zoology and Genetics | |
dc.date | 2018-06-12T12:29:05.000 | |
dc.date.accessioned | 2020-07-07T05:16:52Z | |
dc.date.available | 2020-07-07T05:16:52Z | |
dc.date.copyright | Thu Jan 01 00:00:00 UTC 1998 | |
dc.date.issued | 1998 | |
dc.description.abstract | <p>G4-DNA is a four-stranded structure that is formed by guanine-rich sequences. We report here the purification and characterization of a novel G4-DNA binding protein from <em>Tetrahymena thermophila</em>, designated TGP2. TGP2 was found to preferentially bind to G4-DNA oligonucleotides with adjacent single-stranded domains containing phosphorylated 5‘ ends and the sequence element, 5‘-ACTG-3‘. The amino acid sequence of TGP2 has high similarity to dihydrolipoamide dehydrogenase (DLDH) from a variety of species, and TGP2 was shown to have DLDH activity. Purified DLDH from porcine heart and bovine intestinal mucosa were shown to bind specifically to G4-DNA oligonucleotides. On the basis of these results we conclude that TGP2 is DLDH in <em>T. thermophila</em> and suggest that the G4-DNA binding capability of TGP2/DLDH may be biologically relevant.</p> | |
dc.description.comments | <p>Reprinted (adapted) with permission from A Tetrahymena thermophila G4-DNA Binding Protein with Dihydrolipoamide Dehydrogenase Activity. Kehkooi Kee, Luming Niu, and Eric Henderson. <em>Biochemistry</em> <strong>1998</strong> <em>37</em> (12), 4224-4234. DOI: 10.1021/bi9716377. Copyright 1998 American Chemical Society.</p> | |
dc.format.mimetype | application/pdf | |
dc.identifier | archive/lib.dr.iastate.edu/zool_pubs/27/ | |
dc.identifier.articleid | 1027 | |
dc.identifier.contextkey | 10132874 | |
dc.identifier.s3bucket | isulib-bepress-aws-west | |
dc.identifier.submissionpath | zool_pubs/27 | |
dc.identifier.uri | https://dr.lib.iastate.edu/handle/20.500.12876/92636 | |
dc.language.iso | en | |
dc.source.bitstream | archive/lib.dr.iastate.edu/zool_pubs/27/0-Permission_for_ACS_1998_A_Tetrahymena.pdf|||Fri Jan 14 23:05:36 UTC 2022 | |
dc.source.bitstream | archive/lib.dr.iastate.edu/zool_pubs/27/1998_Henderson_TetrahymenaThermophila.pdf|||Fri Jan 14 23:05:37 UTC 2022 | |
dc.source.uri | 10.1021/bi9716377 | |
dc.subject.disciplines | Biochemistry | |
dc.subject.disciplines | Cell and Developmental Biology | |
dc.subject.disciplines | Genetics and Genomics | |
dc.subject.disciplines | Molecular Biology | |
dc.subject.disciplines | Molecular Genetics | |
dc.title | A Tetrahymena thermophila G4-DNA Binding Protein with Dihydrolipoamide Dehydrogenase Activity | |
dc.type | article | |
dc.type.genre | article | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 68bc511a-afd6-4353-ae20-066de33682b9 | |
relation.isOrgUnitOfPublication | 4a2929da-5374-4338-b62f-f5fd9e156ef9 |
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