Cytosolic Ascorbate Peroxidase 1 Is a Central Component of the Reactive Oxygen Gene Network of Arabidopsis

dc.contributor.author Davletova, Sholpan
dc.contributor.author Oliver, David
dc.contributor.author Rizshsky, Ludmila
dc.contributor.author Liang, Hongjian
dc.contributor.author Zhong, Shengqiang
dc.contributor.author Oliver, David
dc.contributor.author Coutu, Jesse
dc.contributor.author Shulaev, Vladimir
dc.contributor.author Schlauch, Karen
dc.contributor.author Mittler, Ron
dc.contributor.department Genetics, Development and Cell Biology
dc.date 2018-02-13T10:26:06.000
dc.date.accessioned 2020-06-30T04:02:43Z
dc.date.available 2020-06-30T04:02:43Z
dc.date.copyright Sat Jan 01 00:00:00 UTC 2005
dc.date.embargo 2013-05-03
dc.date.issued 2005-01-01
dc.description.abstract <p>Reactive oxygen species (ROS), such as O<sub>2</sub><sup>−</sup> and H<sub>2</sub>O<sub>2</sub>, play a key role in plant metabolism, cellular signaling, and defense. In leaf cells, the chloroplast is considered to be a focal point of ROS metabolism. It is a major producer of O<sub>2</sub><sup>−</sup>and H<sub>2</sub>O<sub>2</sub> during photosynthesis, and it contains a large array of ROS-scavenging mechanisms that have been extensively studied. By contrast, the function of the cytosolic ROS-scavenging mechanisms of leaf cells is largely unknown. In this study, we demonstrate that in the absence of the cytosolic H<sub>2</sub>O<sub>2</sub>-scavenging enzyme ascorbate peroxidase 1 (APX1), the entire chloroplastic H<sub>2</sub>O<sub>2</sub>-scavenging system of <em>Arabidopsis thaliana</em> collapses, H<sub>2</sub>O<sub>2</sub> levels increase, and protein oxidation occurs. We further identify specific proteins oxidized in APX1-deficient plants and characterize the signaling events that ensue in knockout-<em>Apx1</em> plants in response to a moderate level of light stress. Using a dominant-negative approach, we demonstrate that heat shock transcription factors play a central role in the early sensing of H<sub>2</sub>O<sub>2</sub> stress in plants. Using knockout plants for the NADPH oxidase D protein (knockout-<em>RbohD</em>), we demonstrate that RbohD might be required for ROS signal amplification during light stress. Our study points to a key role for the cytosol in protecting the chloroplast during light stress and provides evidence for cross-compartment protection of thylakoid and stromal/mitochondrial APXs by cytosolic APX1.</p>
dc.description.comments <p>This article is from <em>The Plant Cell </em>17, no. 1 (January 2005): 268–281, doi:<a href="http://dx.doi.org/10.1105/tpc.104.026971" target="_blank">10.1105/tpc.104.026971</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/gdcb_las_pubs/7/
dc.identifier.articleid 1006
dc.identifier.contextkey 4106501
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath gdcb_las_pubs/7
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/37981
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/gdcb_las_pubs/7/2005_DavletovaS_CytosolicAscorbatePeroxidase.pdf|||Sat Jan 15 01:31:59 UTC 2022
dc.source.uri 10.​1105/​tpc.​104.​026971
dc.subject.disciplines Agriculture
dc.subject.disciplines Botany
dc.subject.disciplines Developmental Biology
dc.title Cytosolic Ascorbate Peroxidase 1 Is a Central Component of the Reactive Oxygen Gene Network of Arabidopsis
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication b1c85429-ff9e-4a99-9913-9ee528472b63
relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
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