Polycaprolactone Microfibrous Scaffolds to Navigate Neural Stem Cells

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dc.contributor.author Sakaguchi, Donald
dc.contributor.author Sharifi, Farrokh
dc.contributor.author Patel, Bhavika
dc.contributor.author Dzuilko, Adam
dc.contributor.author Montazami, Reza
dc.contributor.author Sakaguchi, Donald
dc.contributor.author Hashemi, Nicole
dc.contributor.author Hashemi, Nastaran
dc.contributor.department Mechanical Engineering
dc.contributor.department Neuroscience
dc.contributor.department Genetics, Development and Cell Biology
dc.date 2018-02-18T11:53:22.000
dc.date.accessioned 2020-06-30T04:01:23Z
dc.date.available 2020-06-30T04:01:23Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2016
dc.date.issued 2016-01-01
dc.description.abstract <p>Fibrous scaffolds have shown promise in tissue engineering due to their ability to improve cell alignment and migration. In this paper, poly(ε-caprolactone) (PCL) fibers are fabricated in different sizes using a microfluidic platform. By using this approach, we demonstrated considerable flexibility in ability to control the size of the fibers. It was shown that the average diameter of the fibers was obtained in the range of 2.6–36.5 μm by selecting the PCL solution flow rate from 1 to 5 μL min–1 and the sheath flow rate from 20 to 400 μL min–1 in the microfluidic channel. The microfibers were used to create 3D microenvironments in order to investigate growth and differentiation of adult hippocampal stem/progenitor cells (AHPCs) in vitro. The results indicated that the 3D topography of the PCL substrates, along with chemical (extracellular matrix) guidance cues supported the adhesion, survival, and differentiation of the AHPCs. Additionally, it was found that the cell deviation angle for 44–66% of cells on different types of fibers was less than 10°. This reveals the functionality of PCL fibrous scaffolds for cell alignment important in applications such as reconnecting serious nerve injuries and guiding the direction of axon growth as well as regenerating blood vessels, tendons, and muscle tissue.</p>
dc.description.comments <p>This article is from <em>Biomacromolecules </em>17 (2016): 3287, doi: <a href="http://dx.doi.org/10.1021/acs.biomac.6b01028" target="_blank">10.1021/acs.biomac.6b01028</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/gdcb_las_pubs/146/
dc.identifier.articleid 1144
dc.identifier.contextkey 10198019
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath gdcb_las_pubs/146
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/37813
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/gdcb_las_pubs/146/0-Permission_for_2016_Polycaprolactone.pdf|||Fri Jan 14 20:23:09 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/gdcb_las_pubs/146/2016_Sakaguchi_PolycaprolactoneMicrofibrous.pdf|||Fri Jan 14 20:23:10 UTC 2022
dc.source.uri 10.1021/acs.biomac.6b01028
dc.subject.disciplines Cell Anatomy
dc.subject.disciplines Cell Biology
dc.subject.disciplines Mechanical Engineering
dc.title Polycaprolactone Microfibrous Scaffolds to Navigate Neural Stem Cells
dc.type article
dc.type.genre article
dspace.entity.type Publication
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