Gene expression in hypothalamus, liver, and adipose tissues and food intake response to melanocortin-4 receptor agonist in pigs expressing melanocortin-4 receptor mutations

dc.contributor.author Barb, C. Richard
dc.contributor.author Hausman, Gary
dc.contributor.author Rekaya, Romdhane
dc.contributor.author Lents, Clay
dc.contributor.author Lkhagvadorj, Sender
dc.contributor.author Qu, Long
dc.contributor.author Cai, Weiguo
dc.contributor.author Couture, Oliver
dc.contributor.author Anderson, Lloyd
dc.contributor.author Dekkers, Jack
dc.contributor.author Tuggle, Christopher
dc.contributor.department Animal Science
dc.contributor.department Statistics
dc.contributor.department Bioinformatics and Computational Biology
dc.date 2018-02-17T11:09:25.000
dc.date.accessioned 2020-06-29T23:39:04Z
dc.date.available 2020-06-29T23:39:04Z
dc.date.issued 2010-05-01
dc.description.abstract <p>Transcriptional profiling was used to identify genes and pathways that responded to intracerebroventricular injection of melanocortin-4 receptor (MC4R) agonist [Nle4, D-Phe7]-α-melanocyte stimulating hormone (NDP-MSH) in pigs homozygous for the missense mutation in the MC4R, D298 allele (<em>n</em> = 12), N298 allele (<em>n</em> = 12), or heterozygous (<em>n</em> = 12). Food intake (FI) was measured at 12 and 24 h after treatment. All pigs were killed at 24 h after treatment, and hypothalamus, liver, and back-fat tissue was collected. NDP-MSH suppressed (<em>P</em> < 0.004) FI at 12 and 24 h in all animals after treatment. In response to NDP-MSH, 278 genes in hypothalamus (<em>q</em> ≤ 0.07, <em>P</em> ≤ 0.001), 249 genes in liver (<em>q</em> ≤ 0.07, <em>P</em> ≤ 0.001), and 5,066 genes in fat (<em>q</em> ≤ 0.07, <em>P</em> ≤ 0.015) were differentially expressed. Pathway analysis of NDP-MSH-induced differentially expressed genes indicated that genes involved in cell communication, nucleotide metabolism, and signal transduction were prominently downregulated in the hypothalamus. In both liver and adipose tissue, energy-intensive biosynthetic and catabolic processes were downregulated in response to NDP-MSH. This included genes encoding for biosynthetic pathways such as steroid and lipid biosynthesis, fatty acid synthesis, and amino acid synthesis. Genes involved in direct energy-generating processes, such as oxidative phosphorylation, electron transport, and ATP synthesis, were upregulated, whereas TCA-associated genes were prominently downregulated in NDP-MSH-treated pigs. Our data also indicate a metabolic switch toward energy conservation since genes involved in energy-intensive biosynthetic and catabolic processes were downregulated in NDP-MSH-treated pigs.</p>
dc.description.comments <p>This article is from <em>Physiological Genomics</em> 41 (2010): 254–268, doi:<a href="http://dx.doi.org/10.1152/physiolgenomics.00006.2010" target="_blank">10.1152/physiolgenomics.00006.2010</a>.</p>
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dc.identifier archive/lib.dr.iastate.edu/ans_pubs/217/
dc.identifier.articleid 1215
dc.identifier.contextkey 8046079
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/217
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/9622
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/217/2010_Barb_GeneExpression.pdf|||Fri Jan 14 22:38:43 UTC 2022
dc.source.uri 10.1152/physiolgenomics.00006.2010
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Bioinformatics
dc.subject.disciplines Biometry
dc.subject.disciplines Computational Biology
dc.subject.disciplines Computational Neuroscience
dc.title Gene expression in hypothalamus, liver, and adipose tissues and food intake response to melanocortin-4 receptor agonist in pigs expressing melanocortin-4 receptor mutations
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
relation.isOrgUnitOfPublication 264904d9-9e66-4169-8e11-034e537ddbca
relation.isOrgUnitOfPublication c331f825-0643-499a-9eeb-592c7b43b1f5
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