Mobile mRNA: a long distance signaling system in potato

dc.contributor.advisor David J. Hannapel
dc.contributor.author Lin, Tian
dc.contributor.department Horticulture
dc.date 2018-07-21T11:22:28.000
dc.date.accessioned 2020-06-30T02:53:44Z
dc.date.available 2020-06-30T02:53:44Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2014
dc.date.embargo 2015-07-30
dc.date.issued 2014-01-01
dc.description.abstract <p>Mobile mRNA is one of the most important long-distance signals in plant vascular system. They play important roles in responding to environmental cues, such as photoperiod. There are numerous studies on mobile mRNAs in plants and thousand of mRNAs have been detected in phloem sap. One of the BEL1-like genes, designated StBEL5, has transcripts that move long distance from leaf to stolon tips and enhance tuberization. However, not many mobile mRNAs have been characterized. The mechanism of the long-distance movement of mRNAs is not clear. In this dissertation, we conducted research on different levels to characterize known mobile mRNA and to explore unknown mobile mRNAs. We verified movement of StBEL5 RNA to roots correlated with increased growth, changes in morphology, and accumulation of GA2-oxidase1, YUCCA1, and isopentenyl transferase transcripts. Making use of the potato genome and current experimental data, a comprehensive profile of the StBEL family is presented in this study as well. Combining Laser Capture Microdissection and RNA-seq, we provide a dynamic approach to the study of phloem tissue and a comprehensive picture of the mechanisms associated with long-distance signaling. We identified 17- and 18k transcripts in the phloem-associated tissue of petiole and stem in potato, respectively. These data provide valuable insights into potentially novel phloem-mobile RNAs and phloem-associated RNA-binding proteins.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/14012/
dc.identifier.articleid 5019
dc.identifier.contextkey 6199739
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/14012
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/28199
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/14012/Lin_iastate_0097E_14428.pdf|||Fri Jan 14 20:12:13 UTC 2022
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Molecular Biology
dc.subject.disciplines Plant Biology
dc.subject.disciplines Plant Sciences
dc.subject.keywords LCM
dc.subject.keywords phloem
dc.subject.keywords RNA binding proteins
dc.subject.keywords RNA-seq
dc.subject.keywords StBEL5
dc.subject.keywords tuberization
dc.title Mobile mRNA: a long distance signaling system in potato
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication df043cd4-424c-49f5-8685-318972aae642
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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