A light and electron microscopic study of the development of antheridia in Onoclea sensibilis L

dc.contributor.author Kotenko, Jane
dc.contributor.department Botany
dc.date 2018-08-16T17:27:47.000
dc.date.accessioned 2020-07-02T06:07:17Z
dc.date.available 2020-07-02T06:07:17Z
dc.date.copyright Sat Jan 01 00:00:00 UTC 1983
dc.date.issued 1983
dc.description.abstract <p>Axenic cultures of Onoclea sensibilis L. form antheridia when treated with Antheridiogen Pt (A(,pt)). A dilute concentration of calcium hypochlorite, in contact with spores for less than 2 minutes, inhibits the rate of vegetative cell divisions, but has little affect on gametophyte morphology or antheridium development. The A(,pt) inhibits the rate of vegetative cell divisions to the same extent as calcium hypochlorite, but the effects are not synergistic;The maximum and minimum induction periods of antheridium formation are 5- 1/2 and 2- 1/2 days when treated on the day of sowing and 4 days later, respectively. The number of antheridia produced is highly correlated to the number of vegetative cells present per gametophyte;The division phase of antheridium development takes about 4 days. The differentiation of spermatids into spermatozoids takes about 3 days;First-formed antheridia occur on lateral, marginal cells and later-formed antheridia occur on upper, central cells. Only a portion of vegetative cells ever produce antheridia;Antheridium initials form at the anterior end of elongate, vegetative cells by an asymmetric cell division preceded by nuclear migration and an accumulation of RNA-staining cytoplasm in the anterior region. Prior to the first two cell divisions in the expanded initial, the cytoplasm becomes polarly distributed. A densely cytoplasmic, spermatogenous cell becomes enclosed by three vacuolate, jacket cells. The orientation of the divisions of the jacket cell walls is consistent with the classical theory of antheridium development. The daughter cells of the first two jacket cell divisions are different in shape, but similar in volume;Antheridia with 32 and 64 spermatids occur on gametophytes with equal frequency. The volume of each spermatid is similar regardless of the number present. The early stage in blepharoplast formation consists of a sphere of flocculent material containing two tri-laminate plaques oriented parallel to each other;During spermatid differentiation, two successive layers of cell wall material are deposited; the first layer during the rounding up of the spermatids, the second layer just before the spermatids decrease in volume and separate from the cell wall. Spermatozoids are released ensheathed in a layer of cell wall material.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/8494/
dc.identifier.articleid 9493
dc.identifier.contextkey 6335132
dc.identifier.doi https://doi.org/10.31274/rtd-180813-8587
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/8494
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/81488
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/8494/r_8407093_Redacted.pdf|||Sat Jan 15 02:12:06 UTC 2022
dc.subject.disciplines Botany
dc.subject.keywords Botany
dc.title A light and electron microscopic study of the development of antheridia in Onoclea sensibilis L
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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