Microbial assemblages and methanogenesis pathways impact methane production and foaming in manure deep-pit storages

Date
2021-08-03
Authors
Yang, Fan
Andersen, Daniel
Trabue, Steven
Kent, Angela
Pepple, Laura
Gates, Richard
Howe, Adina
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Research Projects
Organizational Units
Journal Issue
Series
Abstract

Foam accumulation in swine manure deep-pits has been linked to explosions and flash fires that pose devastating threats to humans and livestock. It is clear that methane accumulation within these pits is the fuel for the fire; it is not understood what microbial drivers cause the accumulation and stabilization of methane. Here, we conducted a 13-month field study to survey the physical, chemical, and biological changes of pit-manure across 46 farms in Iowa. Our results showed that an increased methane production rate was associated with less digestible feed ingredients, suggesting that diet influences the storage pit’s microbiome. Targeted sequencing of the bacterial 16S rRNA and archaeal mcrA genes was used to identify microbial communities’ role and influence. We found that microbial communities in foaming and non-foaming manure were significantly different, and that the bacterial communities of foaming manure were more stable than those of non-foaming manure. Foaming manure methanogen communities were enriched with uncharacterized methanogens whose presence strongly correlated with high methane production rates. We also observed strong correlations between feed ration, manure characteristics, and the relative abundance of specific taxa, suggesting that manure foaming is linked to microbial community assemblage driven by efficient free long-chain fatty acid degradation by hydrogenotrophic methanogenesis.

Description

This article is published as Yang, Fan, Daniel S. Andersen, Steven Trabue, Angela D. Kent, Laura M. Pepple, Richard S. Gates, and Adina S. Howe. "Microbial assemblages and methanogenesis pathways impact methane production and foaming in manure deep-pit storages." PLoS ONE 16, no. 8 (2021): e0254730. DOI: 10.1371/journal.pone.0254730.

Keywords
Citation
DOI
Collections