Comparative Proteomics of Rubber Latex Revealed Multiple Protein Species of REF/SRPP Family Respond Diversely to Ethylene Stimulation among Different Rubber Tree Clones

dc.contributor.author Li, Ling
dc.contributor.author Tong, Zheng
dc.contributor.author Wurtele, Eve
dc.contributor.author Wang, Dan
dc.contributor.author Sun, Yong
dc.contributor.author Yang, Qian
dc.contributor.author Meng, Xueru
dc.contributor.author Wang, Limin
dc.contributor.author Feng, Weiqiang
dc.contributor.author Li, Ling
dc.contributor.author Wurtele, Eve
dc.contributor.author Wang, Xuchu
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Center for Metabolic Biology
dc.date 2019-09-22T09:46:40.000
dc.date.accessioned 2020-06-30T04:02:02Z
dc.date.available 2020-06-30T04:02:02Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2017
dc.date.issued 2017-01-01
dc.description.abstract <p>Rubber elongation factor (REF) and small rubber particle protein (SRPP) are two key factors for natural rubber biosynthesis. To further understand the roles of these proteins in rubber formation, six different genes for latex abundant REF or SRPP proteins, including REF<sub>138,175,258</sub> and SRPP<sub>117,204,243</sub>, were characterized from Hevea brasiliensis Reyan (RY) 7-33-97. Sequence analysis showed that REFs have a variable and long N-terminal, whereas SRPPs have a variable and long C-terminal beyond the REF domain, and REF<sub>258</sub> has a β subunit of ATPase in its N-terminal. Through two-dimensional electrophoresis (2-DE), each REF/SRPP protein was separated into multiple protein spots on 2-DE gels, indicating they have multiple protein species. The abundance of REF/SRPP proteins was compared between ethylene and control treatments or among rubber tree clones with different levels of latex productivity by analyzing 2-DE gels. The total abundance of each REF/SRPP protein decreased or changed a little upon ethylene stimulation, whereas the abundance of multiple protein species of the same REF/SRPP changed diversely. Among the three rubber tree clones, the abundance of the protein species also differed significantly. Especially, two protein species of REF<sub>175</sub> or REF<sub>258</sub> were ethylene-responsive only in the high latex productivity clone RY 8-79 instead of in RY 7-33-97 and PR 107. Some individual protein species were positively related to ethylene stimulation and latex productivity. These results suggested that the specific protein species could be more important than others for rubber production and post-translational modifications might play important roles in rubber biosynthesis.</p>
dc.description.comments <p>This article is published as Tong, Zheng, Dan Wang, Yong Sun, Qian Yang, Xueru Meng, Limin Wang, Weiqiang Feng, Ling Li, Eve Wurtele, and Xuchu Wang. "Comparative proteomics of rubber latex revealed multiple protein species of REF/SRPP family respond diversely to ethylene stimulation among different rubber tree clones." <em>International journal of molecular sciences</em> 18, no. 5 (2017): 958. doi: <a href="https://doi.org/10.3390/ijms18050958">10.3390/ijms18050958</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/gdcb_las_pubs/226/
dc.identifier.articleid 1230
dc.identifier.contextkey 15130244
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath gdcb_las_pubs/226
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/37901
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/gdcb_las_pubs/226/2017_Li_ComparativeProteomics.pdf|||Fri Jan 14 22:44:00 UTC 2022
dc.source.uri 10.3390/ijms18050958
dc.subject.disciplines Cell and Developmental Biology
dc.subject.disciplines Genetics
dc.subject.disciplines Molecular Genetics
dc.subject.keywords comparative proteomics
dc.subject.keywords Hevea brasiliensis
dc.subject.keywords ethylene stimulation
dc.subject.keywords latex productivity
dc.subject.keywords rubber elongation factor
dc.subject.keywords small rubber particle protein
dc.title Comparative Proteomics of Rubber Latex Revealed Multiple Protein Species of REF/SRPP Family Respond Diversely to Ethylene Stimulation among Different Rubber Tree Clones
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
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