Development of a Real-Time Polymerase Chain Reaction Detection Method for the Quantitation of Myeloperoxidase Transcripts in Porcine Tissues

dc.contributor.author Fitzsimmons, C.
dc.contributor.author Marklund, L.
dc.contributor.author Tuggle, C.
dc.contributor.author Stabel, T.
dc.date 2018-02-12T23:58:30.000
dc.date.accessioned 2020-07-02T07:01:05Z
dc.date.available 2020-07-02T07:01:05Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2001
dc.date.embargo 2012-08-13
dc.date.issued 2001-01-01
dc.description.abstract <p>Myeloperoxidase (MPO) is a hemoprotein present in azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. It catalyzes the oxidation of halide ions to their respective hypohalous acids, which are used for microbial killing by phagocytic cells. Measurement of MPO activity is often used as a marker of neutrophil infiltration into tissues. We have designed a quantitative reverse transcription-polymerase chain reaction detection method for porcine MPO transcripts by using TaqMan realtime PCR technology. Total RNA was isolated from lung and spleen tissue collected 7 days post-intranasal inoculation with <em>Salmonella choleraesuis</em> (n=4) or saline (n=4). The lung and spleen samples were pooled before RNA isolation to create uninfected control and infected RNAs for each tissue. Expression of MPO mRNA was highest in infected spleen (12.5 ± 2.85 relative units (RU)), followed by the uninfected spleen sample (2.9 ± 1.50 RU). There was no difference in MPO expression between uninfected and infected lung samples. In conclusion, levels of MPO mRNA expression in porcine spleen and lung indicate a differential response to infection between the two tissues. This difference may be associated with bacterialhost adaptation of <em>S. choleraesuis</em>. The TaqMan assay for MPO also can be used to discover tissue-specific responses between individuals or groups of pigs exhibiting distinct phenotypic responses to infection.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/swinereports_2000/20/
dc.identifier.articleid 1021
dc.identifier.contextkey 3210190
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath swinereports_2000/20
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91241
dc.language.iso en
dc.relation.ispartofseries Animal Science Research Reports
dc.relation.ispartofseries ASL R665
dc.source.bitstream archive/lib.dr.iastate.edu/swinereports_2000/20/asl_665.pdf|||Fri Jan 14 22:16:18 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.keywords ASL R665
dc.title Development of a Real-Time Polymerase Chain Reaction Detection Method for the Quantitation of Myeloperoxidase Transcripts in Porcine Tissues
dc.type article
dc.type.genre breeding_physiology
dspace.entity.type Publication
relation.isSeriesOfPublication 7f3839b7-b833-4418-a6fa-adda2b23950a
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