Evolution of the iss Gene in Escherichia coli

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dc.contributor.author Johnson, Timothy
dc.contributor.author Nolan, Lisa
dc.contributor.author Wannemuehler, Yvonne
dc.contributor.author Nolan, Lisa
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-13T10:37:28.000
dc.date.accessioned 2020-07-07T05:14:45Z
dc.date.available 2020-07-07T05:14:45Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2008
dc.date.embargo 2013-05-06
dc.date.issued 2008-04-01
dc.description.abstract <p>The increased serum survival gene <em>iss</em> has long been recognized for its role in extraintestinal pathogenic <em>Escherichia coli</em> (ExPEC) virulence. <em>iss</em> has been identified as a distinguishing trait of avian ExPEC but not of human ExPEC. This gene has been localized to large virulence plasmids and shares strong similarities with the <em>bor</em> gene from bacteriophage λ. Here, we demonstrate that three alleles of <em>iss</em> occur among <em>E. coli</em> isolates that appear to have evolved from a common λ<em>bor</em> precursor. In addition to the occurrence of <em>iss</em> on the ColV/BM virulence plasmids, at least two <em>iss</em> alleles occur within the <em>E. coli</em> chromosome. One of these alleles (designated type 3) was found to occur in the genomes of all currently sequenced ExPEC strains on a similar prophage element that also harbors the Sit iron and manganese transport system. When the prevalence of the three <em>iss</em> types was examined among 487 <em>E. coli</em> isolates, the <em>iss</em> type 3 gene was found to occur at a high frequency among ExPEC isolates, irrespective of the host source. The plasmid-borne <em>iss</em> allele (designated type 1) was highly prevalent among avian pathogenic <em>E. coli</em> and neonatal meningitis-associated <em>E. coli</em>isolates but not among uropathogenic <em>E. coli</em> isolates. This study demonstrates the evolution of <em>iss</em> in <em>E. coli</em> and provides an additional tool for discriminating among <em>E. coli</em> pathotypes through the differentiation of the three <em>iss</em> allele types and <em>bor</em>.</p>
dc.description.comments <p>This article is from <em>Applied and Environmental Microbiology</em> 74, no. 8 (April 2008): 2360–2369, doi:<a href="http://dx.doi.org/10.1128/AEM.02634-07" target="_blank">10.1128/AEM.02634-07</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/17/
dc.identifier.articleid 1015
dc.identifier.contextkey 4110051
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/17
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92276
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/17/2008_JohnsonTJ_EvolutionissGene.pdf|||Fri Jan 14 21:09:13 UTC 2022
dc.source.uri 10.1128/​AEM.02634-07
dc.subject.disciplines Genomics
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Evolution of the iss Gene in Escherichia coli
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 9e7506b4-e945-47cf-9195-e814dac6c9fd
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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