Increasing Seed Viability of Maize Haploid Inducing Lines by Genetic and Non-Genetic Approaches

dc.contributor.author Irani, Sayareh
dc.contributor.author Knapp, Allen
dc.contributor.author Lubberstedt, Thomas
dc.contributor.author Lubberstedt, Thomas
dc.contributor.author Frei, Ursula
dc.contributor.author Askari, Ehsan
dc.contributor.department Agronomy
dc.date 2018-03-01T16:57:15.000
dc.date.accessioned 2020-06-29T23:05:17Z
dc.date.available 2020-06-29T23:05:17Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2016
dc.date.issued 2016-01-01
dc.description.abstract <p>Some haploid inducing lines used in the production of maize doubled haploids (DHs), express germination problems and reduced vigor. In this study, haploid inducing lines RWS, RWK-76 and their reciprocal hybrids RWS/RWK-76 and RWK-76/RWS were examined for viability by Tetrazolium (TZ) and germination ability by standard germination tests. Evaluation based on TZ tests showed that 59% of the seed of RWK-76/RWS were not viable, compared with only 12% dead seed in RWS/RWK-76. Similarly, the percentage and speed of germination in RWK- 76/RWS (25%, 1.53) was lower than for RWS/RWK-76 (74%, 4.30). In an effort to develop a quick method for assessing seed viability in these lines, the TZ test was repeated in a different way. Seed from each genotype was placed in beakers containing distilled water.Seed would either float or sink. Subsequent TZ testing confirmed that seed that floated was dead, and seed that sank was alive, although some of them had defective embryos. The dead seed in both genotypes failed to develop an embryo, leaving an empty cavity that would fill with air and cause seed to float on water. This feature can be exploited for a simple and practical method to separate living from dead seed. In addition, we surveyed theig1 (indeterminate gametophyte) gene as a candidate for germination problems in inducer lines.Sequencing data from theig1 region showed that RWS and RWK-76 differed in one nucleotide and amino acidin the firstexon of ig1.Segregation of ig1 alleles from RWS and RWK-76 wassignificantly (P=0.01) distortedin the respectiveF2 population relative to the expected Mendelian segregation ratio (1:2:1). Thus, either ig1 or a linked gene in the ig1 region affect seed viability.</p>
dc.description.comments <p>This is a manuscript of an article published as Irani, Sayareh, Allen D. Knapp, Thomas Lubberstedt, Ursula Frei, and Ehsan Askari. "Increasing Seed Viability of Maize Haploid Inducing Lines by Genetic and Non-Genetic Approaches." <em>Crop Science</em> 56, no. 4 (2016): 1940-1947. doi: <a href="http://dx.doi.org/10.2135/cropsci2015.11.0713" target="_blank">10.2135/cropsci2015.11.0713</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/agron_pubs/444/
dc.identifier.articleid 1493
dc.identifier.contextkey 11667688
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath agron_pubs/444
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/4803
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/agron_pubs/444/2016_Lubberstedt_IncreasingSeedManuscript.pdf|||Sat Jan 15 00:18:46 UTC 2022
dc.source.uri 10.2135/cropsci2015.11.0713
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Plant Breeding and Genetics
dc.title Increasing Seed Viability of Maize Haploid Inducing Lines by Genetic and Non-Genetic Approaches
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 4e4330cd-db15-4ac5-8924-41119139cf32
relation.isOrgUnitOfPublication fdd5c06c-bdbe-469c-a38e-51e664fece7a
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