Programmable DNA Nanosystem for Molecular Interrogation

Date
2016-06-07
Authors
Mathur, Davita
Henderson, Eric
Henderson, Eric
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Abstract

We describe a self-assembling DNA-based nanosystem for interrogating molecular interactions. The nanosystem contains a rigid supporting dumbbell-shaped frame, a cylindrical central core, and a mobile ring that is coaxial with the core. Motion of the ring is influenced by several control elements whose force-generating capability is based on the transition of single-stranded DNA to double-stranded DNA. These forces can be directed to act in opposition to adhesive forces between the ring and the frame thereby providing a mechanism for molecular detection and interrogation at the ring-frame interface. As proof of principle we use this system to evaluate base stacking adhesion and demonstrate detection of a soluble nucleic acid viral genome mimic.

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<p>This article is from <em>Scientific Reports </em>6 (2016): 27413, doi: <a href="http://dx.doi.org/10.1038/srep27413" target="_blank">10.1038/srep27413</a>.</p>
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