Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system ‘FocusField2-6GallupqPCRSet-upTool-001’ to attain consistently high fidelity qPCR reactions
dc.contributor.author | Gallup, Jack | |
dc.contributor.author | Ackermann, Mark | |
dc.contributor.author | Ackermann, Mark | |
dc.contributor.department | Veterinary Pathology | |
dc.date | 2018-02-13T06:19:37.000 | |
dc.date.accessioned | 2020-07-07T05:16:13Z | |
dc.date.available | 2020-07-07T05:16:13Z | |
dc.date.copyright | Sun Jan 01 00:00:00 UTC 2006 | |
dc.date.embargo | 2013-02-25 | |
dc.date.issued | 2006-12-01 | |
dc.description.abstract | <p>The purpose of this manuscript is to discuss fluorogenic real-time quantitative polymerase chain reaction (qPCR) inhibition and to introduce/define a novel Microsoft Excel-based file system which provides a way to detect and avoid inhibition, and enables investigators to consistently design dynamically-sound, truly LOG-linear qPCR reactions very quickly. The qPCR problems this invention solves are universal to all qPCR reactions, and it performs all necessary qPCR set-up calculations in about 52 seconds (using a pentium 4 processor) for up to seven qPCR targets and seventytwo samples at a time - calculations that commonly take capable investigators days to finish. We have named this custom Excel-based file system “FocusField2-6GallupqPCRSet-upTool-001” (FF2-6-001 qPCR set-up tool), and are in the process of transforming it into professional qPCR set-up software to be made available in 2007. The current prototype is already fully functional.</p> | |
dc.description.comments | <p>This article is from <em>Biological Procedures Online </em>8, no. 1 ( December 2006): 87–153, doi:<a href="http://dx.doi.org/10.1251/bpo122" target="_blank">10.1251/bpo122</a>.</p> | |
dc.format.mimetype | application/pdf | |
dc.identifier | archive/lib.dr.iastate.edu/vpath_pubs/9/ | |
dc.identifier.articleid | 1007 | |
dc.identifier.contextkey | 3783357 | |
dc.identifier.s3bucket | isulib-bepress-aws-west | |
dc.identifier.submissionpath | vpath_pubs/9 | |
dc.identifier.uri | https://dr.lib.iastate.edu/handle/20.500.12876/92519 | |
dc.language.iso | en | |
dc.source.bitstream | archive/lib.dr.iastate.edu/vpath_pubs/9/2006_Gallup_AddressingFluorogenicRealTime.pdf|||Sat Jan 15 02:20:05 UTC 2022 | |
dc.source.uri | 10.1251/bpo122 | |
dc.subject.disciplines | Veterinary Pathology and Pathobiology | |
dc.subject.keywords | Reverse Transcription | |
dc.subject.keywords | DNA Complementary | |
dc.title | Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system ‘FocusField2-6GallupqPCRSet-upTool-001’ to attain consistently high fidelity qPCR reactions | |
dc.type | article | |
dc.type.genre | article | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | ef246218-d0a8-4526-8d8b-4fac40c21ae7 | |
relation.isAuthorOfPublication | 86c1ed73-b60d-48ce-8f35-449bc320a693 | |
relation.isOrgUnitOfPublication | cf38d7e3-b5f8-4859-83e3-ae8fab6a4c5f |
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