Detection and Identification of Microorganisms in Mixed Cultures by Nanoparticle-Induced NanoSPR Enhanced FTIR Spectroscopy and Chemometrics

dc.contributor.author Wang, Qi
dc.contributor.author Yu, Chenxu
dc.contributor.author Xiao, Nan
dc.contributor.author Yu, Chenxu
dc.contributor.department Agricultural and Biosystems Engineering
dc.date 2018-02-13T16:48:04.000
dc.date.accessioned 2020-06-29T22:39:51Z
dc.date.available 2020-06-29T22:39:51Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2010
dc.date.embargo 2013-10-31
dc.date.issued 2010-01-01
dc.description.abstract <p>Routine identification of pathogenic microorganisms predominantly based on nutritional and biochemical tests is a time-consuming process, but delay may lead to fatal consequences at times. In this work, nanoparticle-induced nanoSPR enhanced IR spectroscopy was used in conjunction with a background elimination data processing algorithm to directly identify microorganisms in mixed cultures. It was demonstrated that the microbial composition of mixtures of different E. coli strains could be accurately identified using our method, at a concentration level of 103 CFU mL-1. The procedure was also applied to determine the presence or absence of pathogenic microorganisms in a simple but real food matrix (apple juice). Results indicated that microorganisms in a cocktail of up to eight different species suspended in an apple juice matrix could be accurately identified for its presence or absence.</p>
dc.description.comments <p>This article is from <em>Transactions of the ASABE</em> 53 (2010): 999–1006, doi:1<a href="http://dx.doi.org/10.13031/2013.30050" target="_blank">0.13031/2013.30050</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/abe_eng_pubs/440/
dc.identifier.articleid 1727
dc.identifier.contextkey 4782430
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath abe_eng_pubs/440
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/1207
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/abe_eng_pubs/440/2010_WangQ_DetectionIdentificationMicroorganisms.pdf|||Sat Jan 15 00:18:08 UTC 2022
dc.source.uri 10.13031/2013.30050
dc.subject.disciplines Agriculture
dc.subject.disciplines Bioresource and Agricultural Engineering
dc.subject.keywords IR absorption
dc.subject.keywords Microorganism identification and differentiation
dc.subject.keywords Nanoparticles
dc.subject.keywords Surface enhanced
dc.title Detection and Identification of Microorganisms in Mixed Cultures by Nanoparticle-Induced NanoSPR Enhanced FTIR Spectroscopy and Chemometrics
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 837654ad-03e3-4004-b198-68d7756bcd27
relation.isOrgUnitOfPublication 8eb24241-0d92-4baf-ae75-08f716d30801
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