Genome-wide association and epistasis studies of Sclerotinia sclerotiorum resistance in soybean

dc.contributor.advisor Asheesh K. Singh Moellers, Tara
dc.contributor.department Agronomy 2018-08-11T13:45:50.000 2020-06-30T03:00:59Z 2020-06-30T03:00:59Z Fri Jan 01 00:00:00 UTC 2016 2001-01-01 2016-01-01
dc.description.abstract <p>Sclerotinia stem rot or white mold (WM) [Sclerotinia sclerotiorum (Lib.) de Bary] is an important fungal disease affecting soybean [Glycine max (L.) Merr.] and causes yield and quality losses. WM is prevalent in cool and moist environments, particularly in the soybean growing regions of Northern United States and Canada. Although sources of complete resistance have not yet been identified, several quantitative trait loci (QTL) for partial resistance have been reported but generally using bi-parental mapping populations. Genome-wide association (GWA) studies have been used to dissect complex disease resistance traits in plants and to identify the genes controlling the expression. WM was assessed in 465 diverse plant introduction accessions from the USDA Soybean Germplasm Core Collection and GWA and epistatic interaction analysis was performed using 36,105 SNPs from the SoySNP50K Illumina Infinium BeadChip to 1) discover sources of WM resistance, 2) identify SNPs associated with WM resistance, and 3) determine putative candidate genes for WM resistance. Phenotyping for WM was done under artificial epiphytotic conditions in both field and greenhouse environments. Forty-five main effects and 18 epistatic interactions associated with WM resistance were identified at different growth stages and for multiple response variables. Together, these explained 7-36% of the phenotypic variation. A wide range of candidate genes were identified at the proximity of peak SNPs, which included a few disease response genes previously reported in the literature. The mode of resistance within these candidate genes varied greatly and included functions such as cell wall structure, hormone signaling, and sugar allocation, revealing the complex nature of WM resistance. Several accessions expressing resistance in all environments were identified.</p>
dc.format.mimetype application/pdf
dc.identifier archive/
dc.identifier.articleid 6046
dc.identifier.contextkey 8882563
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/15039
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 20:34:54 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Plant Pathology
dc.subject.disciplines Plant Sciences
dc.subject.keywords Plant Breeding
dc.subject.keywords association
dc.subject.keywords epistasis
dc.subject.keywords resistance
dc.subject.keywords Sclerotinia sclerotiorum
dc.subject.keywords soybean
dc.subject.keywords white mold
dc.title Genome-wide association and epistasis studies of Sclerotinia sclerotiorum resistance in soybean
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
relation.isOrgUnitOfPublication fdd5c06c-bdbe-469c-a38e-51e664fece7a Plant Breeding thesis Master of Science
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