Rapid Detection of Listeria monocytogenes in Mechanically Separated Turkey Meat

dc.contributor.author Ramos, Ann
dc.contributor.author Wesley, Irene
dc.contributor.author Dickson, James
dc.contributor.author Dickson, James
dc.contributor.author Harmon, Karen
dc.date 2018-02-13T00:30:16.000
dc.date.accessioned 2020-07-02T07:00:14Z
dc.date.available 2020-07-02T07:00:14Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 1998
dc.date.issued 1998
dc.description.abstract <p>The purpose of this study was to determine the level of Listeria spp., especially L. monocytogenes, in mechanically separated turkey (MST) meat. During two trials of 25 samples each, Listeria spp. were selected by using two enrichments (University of Vermont-modified I and II) and plating to selective Palcam agar base. A multiplex polymerase chain reaction (PCR) was used to confirm Listeria isolations. The specificity of the multiplex PCR assay was evaluated with reference strains of Listeria from the National Animal Disease Center (NADC) Culture Collection. The Listeria spp. yields a single 938-bp product whereas L. monocytogenes yields the 938-bp product along with a 174-bp fragment. Results from Trial I and II indicated L. monocytogenes could not be detected by PCR in the UVM enrichment due perhaps to PCR inhibitors present in poultry fats and muscle myoglobin. However the multiplex PCR performed from suspect colonies grown on Palcam indicated 48 out of 54 (89%) of the MST meat harbored Listeria spp. Of those, 32 of 48 (67%) were positive for L. monocytogenes.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/swinereports_1997/39/
dc.identifier.articleid 1038
dc.identifier.contextkey 3233651
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath swinereports_1997/39
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91082
dc.language.iso en
dc.relation.ispartofseries Animal Science Research Reports
dc.relation.ispartofseries ASL R1515
dc.source.bitstream archive/lib.dr.iastate.edu/swinereports_1997/39/asl_1515.pdf|||Fri Jan 14 23:54:19 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.keywords ASL R1515
dc.title Rapid Detection of Listeria monocytogenes in Mechanically Separated Turkey Meat
dc.type article
dc.type.genre health
dspace.entity.type Publication
relation.isAuthorOfPublication 87a4e13f-70ef-4b6a-8f45-e9258086d64a
relation.isSeriesOfPublication 7f3839b7-b833-4418-a6fa-adda2b23950a
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