Performance of a Mycoplasma hyopneumoniae serum ELISA for antibody detection in processing fluids

Date
2022-05
Authors
Magtoto, Ronaldo
Armenta-Leyva, Betsy
Dizon-Magtoto, Precy
Cheng, Ting-Yu
Clavijo, Maria J.
Johnson, Clayton
Lopez, Will
Baum, David
Zimmerman, Jeffrey
Giménez-Lirola, Luis
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American Association of Swine Veterinarians
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Giménez-Lirola, Luis
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Veterinary Diagnostic and Production Animal Medicine
Abstract
The diagnostic performance of a commercial Mycoplasma hyopneumoniae (MHP) serum enzyme-linked immunosorbent assay (ELISA) was evaluated for MHP antibody detection in processing fluids (n = 494) using samples from three commercial swine farms. Based on historical monitoring, one farm was considered MHP positive and two were considered MHP negative. Samples were tested at a 1:10 dilution and diagnostic sensitivities and specificities estimated for specific ELISA sample-to-positive (S:P) cutoffs. At S:P ≥ 0.40, diagnostic sensitivity and specificity were estimated as 97.6% and 100.0%, respectively. Overall, the results suggest that processing fluids can be used for MHP antibody surveillance in breeding herds.
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This article is published as Magtoto, Ronaldo, Betsy Armenta-Leyva, Precy Dizon-Magtoto, Ting-Yu Cheng, Maria J. Clavijo, Clayton Johnson, Will Lopez, David Baum, Jeffrey Zimmerman, and Luis G. Giménez-Lirola. "Performance of a Mycoplasma hyopneumoniae serum ELISA for antibody detection in processing fluids." Journal of Swine Health and Production 30, no. 3 (2022): 165-170. DOI: 10.54846/jshap/1265. Copyright 2022 AASV. Posted with permission.
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