Performance of a Mycoplasma hyopneumoniae serum ELISA for antibody detection in processing fluids
Clavijo, Maria J.
American Association of Swine Veterinarians
Is Version Of
Veterinary Diagnostic and Production Animal Medicine
The diagnostic performance of a commercial Mycoplasma hyopneumoniae (MHP) serum enzyme-linked immunosorbent assay (ELISA) was evaluated for MHP antibody detection in processing fluids (n = 494) using samples from three commercial swine farms. Based on historical monitoring, one farm was considered MHP positive and two were considered MHP negative. Samples were tested at a 1:10 dilution and diagnostic sensitivities and specificities estimated for specific ELISA sample-to-positive (S:P) cutoffs. At S:P ≥ 0.40, diagnostic sensitivity and specificity were estimated as 97.6% and 100.0%, respectively. Overall, the results suggest that processing fluids can be used for MHP antibody surveillance in breeding herds.
This article is published as Magtoto, Ronaldo, Betsy Armenta-Leyva, Precy Dizon-Magtoto, Ting-Yu Cheng, Maria J. Clavijo, Clayton Johnson, Will Lopez, David Baum, Jeffrey Zimmerman, and Luis G. Giménez-Lirola. "Performance of a Mycoplasma hyopneumoniae serum ELISA for antibody detection in processing fluids." Journal of Swine Health and Production 30, no. 3 (2022): 165-170. DOI: 10.54846/jshap/1265. Copyright 2022 AASV. Posted with permission.