Usefulness of Adapted Exotic Maize Lines Developed By Doubled Haploid and Single Seed Descent Methods
Date
2021-09-01
Authors
Santos, Iara Goncalves
Verzegnazzi, Anderson L.
Edwards, Jode
Frei, Ursula K.
Boerman, Nicholas
Zuffo, Leandro Tonello
Pires, Luiz P. M.
de La Fuente, Gerald N.
Lubberstedt, Thomas
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
© Author(s) 2021
Authors
Research Projects
Organizational Units
Organizational Unit
Journal Issue
Is Version Of
Versions
Series
Department
Agronomy
Abstract
Adapted exotic maize (Zea mays L.) germplasm, such as BS39, provides a unique opportunity for broadening the genetic base of U.S. Corn Belt germplasm. In vivo doubled haploid (DH) technology has been used to efficiently exploit exotic germplasm. It can help to purge deleterious recessive alleles. The objectives of this study were to determine the usefulness of BS39-derived inbred lines using both SSD and DH methods, to determine the impact of spontaneous as compared to artificial haploid genome doubling on genetic variance among BS39-derived DH lines, and to identify SNP markers associated with agronomic traits among BS39 inbreds monitored at testcross level. We developed two sets of inbred lines directly from BS39 by DH and SSD methods, named BS39_DH and BS39_SSD. Additionally, two sets were derived from a cross between BS39 and A427 (SHGD donor) by DH and SSD methods, named BS39×A427_DH and BS39×A427_SSD, respectively. Grain yield, moisture, plant height, ear height, stalk lodging, and root lodging were measured to estimate genetic parameters. For genome-wide association (GWAS) analysis, inbred lines were genotyped using Genotype-by-Sequencing (GBS) and Diversity Array Technology Sequencing (DArTSeq). Some BS39-derived inbred lines performed better than elite germplasm inbreds and all sets showed significant genetic variance. The presence of spontaneous haploid genome doubling genes did not affect performance of inbred lines. Five SNPs were significant and three of them located within genes related to plant development or abiotic stresses. These results demonstrate the potential of BS39 to add novel alleles to temperate elite germplasm.
Comments
This is a preprint made available through Research Square at doi:10.21203/rs.3.rs-799789/v1.
This work is licensed under a CC BY 4.0 License.
Description
Keywords
spontaneous doubling,
artificial doubling,
doubled haploid,
single-seed descent