Morphological characteristics of tissue cultured clones of Poa pratensis 'Baron' and their endogenous ethylene and cholorophyll response to infection by Bipolaris sorokiniana
Date
1999
Authors
Anderson, Rosemarie Arlene
Major Professor
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Hodges, Clinton F.
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Abstract
Tissue culture derived (TCD) plants of Poa pratensis 'Baron' were evaluated for morphological changes due to somaclonal variation against a seed derived (SD) plant population. TCD plants also were measured for their ethylene and chlorophyll response to inoculation with Bipolaris sorokiniana as compared with SD plants. Morphological differences occurred in leaf blade width, leaf blade length, sheath length, number of tillers, shoot dry weight, and root dry weight. Only the number of rhizomes per plant did not differ. All TCD and SD leaf blades had similar symptom expression in response to infection. Water-soaked to necrotic lesions were visible 48 h after inoculation, lesions developed yellow halos by 72 h, and the entire leaf blade became progressively more chlorotic by 96 h. TCD and SD control (noninoculated) plants did not differ in endogenous ethylene.
Endogenous ethylene of inoculated leaf blades peaked at 48 h. Ethylene content of inoculated leaf blades of the TCD lines ranged from 1.5X to 2.OX greater than that of inoculated SD leaf blades over the 96 h of the study. The leaf blade chlorophyll content of some TCD plants differed from that of SD plants; two TCD lines had more chlorophyll and one line had less chlorophyll. Inoculated leaf blades of all TCD and SD plants decreased in chlorophyll compared with their respective controls at 96 h. Inoculated leaf blades of one TCD line had a greater loss of chlorophyll than other TCD and SD inoculated leaf blades. The observations suggest that somaclonal variation may alter the morphology and physiology of P. pratensis regenerated from tissue culture.
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thesis