Rapid Communication: TaqI Restriction Fragment Length Polymorphism at the Porcine Transporter Associated with Antigen Processing 2 [TAP2] Locus

Date
1994
Authors
Vaske, D. A.
Liu, H. C.
Rothschild, Max
Larson, R. G.
Warner, Carol
Rothschild, Max
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Animal Science
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Animal Science
Abstract

Source and Description of Probe. A 2.7-kb human cDNA clone for TAP2 was excised from the XbaI site of the plasmid pRSV.5neo (Bahram et al., 1991). Method of Detection. DNA was isolated from whole blood and digested with TuqI. Fragments were separated by agarose gel electrophoresis and transferred to charged nylon membranes. Hybridizations were for 16 to 20 h at 65°C (10% dextran sulfate, 7% SDS, .263 M Na2HP04, 1% BSA, 1 mM EDTA, 100 pglmL sonicated denatured salmon sperm DNA). Final washes were at 65°C in .7x SSC, 5% SDS for 15 to 20 min.

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This is an article from Journal of Animal Science 72 (1994): 798, doi:/1994.723798x. Posted with permission.

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