Molecular mechanism of methionine differentiation in high and low methionine maize lines

dc.contributor.author Ali, Muhammad
dc.contributor.author Scott, Marvin
dc.contributor.author Scott, Marvin
dc.contributor.author Bakht, Jehan
dc.contributor.department Agronomy
dc.date 2018-02-18T15:41:05.000
dc.date.accessioned 2020-06-29T23:03:04Z
dc.date.available 2020-06-29T23:03:04Z
dc.date.copyright Sat Jan 01 00:00:00 UTC 2011
dc.date.issued 2011-05-09
dc.description.abstract <p>Since maize is a primary food stuff for humans and livestock, its amino acid balance is important for proper nutrition. Methionine, an essential amino acid and a primary source of sulfur, is lacking in maize endosperm. Several maize populations were developed through breeding with enhanced methionine content in comparison with normal maize populations. BS31HM (high methionine) and BS31LM (low methionine) maize were among such populations created by the selection from the highest or lowest methionine content population from original BS31 maize. Candidate gene approach was adopted to determine the difference between the two populations at transcript level of the selected genes in the endosperm. The genes selected were mostly expressed in the endosperm and could be involved in enhanced methionine biosynthesis. The selected genes, that is, 15-kDa β-zein, 16-kDa γ-zein, 19-kDa α- zeinB1, 27-kDa γ-zein, 22-kDa α-zein and 18-kDa δ-zein were responsible for coding of endosperm storage proteins when analyzed through RT-PCR. Similarly, expression level relative to the high population (2<sup>-ΔΔct</sup>) values were also calculated for BS31HM and BS31LM, respectively. These values were found as 1 and 0.25, 1 and 0.07, 1 and 0.10, 1 and 0.15, 1 and 0.33, 1 and 0.43 for 27-kDa γ-zein, 22- kDa α-zein, 18-kDa δ-zein, 15-kDa β-zein, 16-kDa γ-zein and 19-kDa α-zeinB1, respectively, in both populations. The p-values were determined by student’s t-test at confidence level of 95%. The expression of 18-kDa δ-gene, 15-kDa β-gene and 16-kDa γ-gene were found to be significant (p < 0.05) in high methionine maize population when compared with low methionine maize population. Non significant (p > 0.05) differences in the expression level of 27-kDa γ-gene, 22-kDa α-gene and 19-kDa α- gene were observed in both HM and LM maize populations. From these results it can be concluded that all zein genes did not show expression equally in high and low methionine maize populations.</p>
dc.description.comments <p>This article is published as Ali, Muhammad, Marvin Paul Scott, and Jehan Bakht. "Molecular mechanism of methionine differentiation in high and low methionine maize lines." <em>African Journal of Biotechnology</em> 10, no. 19 (2011): 3747-3752, doi: <a href="http://dx.doi.org/10.5897/AJB10.2294" target="_blank">10.5897/AJB10.2294</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/agron_pubs/167/
dc.identifier.articleid 1177
dc.identifier.contextkey 10442289
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath agron_pubs/167
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/4496
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/agron_pubs/167/2011_Scott_MolecularMechanism.pdf|||Fri Jan 14 21:04:36 UTC 2022
dc.source.uri 10.5897/AJB10.2294
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.keywords Maize
dc.subject.keywords methionine
dc.subject.keywords zein
dc.subject.keywords storage protein
dc.subject.keywords amino acid
dc.subject.keywords real time PCR
dc.title Molecular mechanism of methionine differentiation in high and low methionine maize lines
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 97acee5f-1291-4c27-8929-a8e1617c411d
relation.isOrgUnitOfPublication fdd5c06c-bdbe-469c-a38e-51e664fece7a
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